From Tol2Kit
(New page: === Construction details === pME-Gal4VP16 was made by PCR amplification of Gal4VP16, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation,...) |
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=== Construction details === | === Construction details === | ||
| − | pME-Gal4VP16 was made by PCR amplification of Gal4VP16, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, G1114T. | + | pME-Gal4VP16 was made by PCR amplification of Gal4VP16 (Gal4 DNA binding domain fused to VP16 transactivation domain), using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, G1114T. |
=== Crudely annotated sequence === | === Crudely annotated sequence === | ||
Revision as of 02:18, 24 March 2007
Construction details
pME-Gal4VP16 was made by PCR amplification of Gal4VP16 (Gal4 DNA binding domain fused to VP16 transactivation domain), using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, G1114T.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
pME-Gal4VP16 sequence
Crude map
Screenshot from Sequencher showing locations of components:
