From Tol2Kit
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pME-Gal4VP16 was made by PCR amplification of Gal4VP16 (Gal4 DNA binding domain fused to VP16 transactivation domain), using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, G1114T. | pME-Gal4VP16 was made by PCR amplification of Gal4VP16 (Gal4 DNA binding domain fused to VP16 transactivation domain), using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, G1114T. | ||
| − | === | + | === Sequence === |
| + | Annotated sequence, Genbank format:<br> | ||
| + | [[pME-Gal4VP16 Genbank]] | ||
| + | |||
FASTA file with the full-length sequence as well as sequences of individual components:<br> | FASTA file with the full-length sequence as well as sequences of individual components:<br> | ||
[[pME-Gal4VP16 sequence]] | [[pME-Gal4VP16 sequence]] | ||
Revision as of 03:47, 19 December 2007
Construction details
pME-Gal4VP16 was made by PCR amplification of Gal4VP16 (Gal4 DNA binding domain fused to VP16 transactivation domain), using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, G1114T.
Sequence
Annotated sequence, Genbank format:
pME-Gal4VP16 Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
pME-Gal4VP16 sequence
Crude map
Screenshot from Sequencher showing locations of components:
