From Tol2Kit
Revision as of 02:16, 24 March 2007 by Chi-bin (talk | contribs) (New page: === Construction details === pME-Gal4VP16 was made by PCR amplification of Gal4VP16, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation,...)
Construction details
pME-Gal4VP16 was made by PCR amplification of Gal4VP16, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, G1114T.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
pME-Gal4VP16 sequence
Crude map
Screenshot from Sequencher showing locations of components:
