From Tol2Kit
Construction details
pME-Gal4VP16 was made by PCR amplification of Gal4VP16 (Gal4 DNA binding domain fused to VP16 transactivation domain), using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, G1114T.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
pME-Gal4VP16 sequence
Crude map
Screenshot from Sequencher showing locations of components:
