From Tol2Kit
(New page: === Construction details === pME-nlsEGFP was made by PCR amplification of an nls-EGFP (nuclear-localized GFP) cassette, using primers that add att sites, followed by a BP reaction. Sequen...) |
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=== Construction details === | === Construction details === | ||
| − | pME-nlsEGFP was made by PCR amplification of an nls-EGFP (nuclear- | + | pME-nlsEGFP was made by PCR amplification of an nls-EGFP (EGFP with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct. |
| − | Sequencing confirms that all bases of the insert are correct. | ||
=== Crudely annotated sequence === | === Crudely annotated sequence === | ||
Revision as of 01:51, 24 March 2007
Construction details
pME-nlsEGFP was made by PCR amplification of an nls-EGFP (EGFP with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
pME-nlsEGFP sequence
Crude map
Screenshot from Sequencher showing locations of components:
