From Tol2Kit
(New page: === Construction details === pME-nlsEGFP was made by PCR amplification of an nls-EGFP (nuclear-localized GFP) cassette, using primers that add att sites, followed by a BP reaction. Sequen...) |
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=== Construction details === | === Construction details === | ||
| − | pME-nlsEGFP was made by PCR amplification of an nls-EGFP (nuclear- | + | pME-nlsEGFP was made by PCR amplification of an nls-EGFP (EGFP with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct. |
| − | Sequencing confirms that all bases of the insert are correct. | + | |
| + | === Sequence === | ||
| + | Annotated sequence, Genbank format:<br> | ||
| + | [[pME-nlsEGFP Genbank]] | ||
| − | |||
FASTA file with the full-length sequence as well as sequences of individual components:<br> | FASTA file with the full-length sequence as well as sequences of individual components:<br> | ||
[[pME-nlsEGFP sequence]] | [[pME-nlsEGFP sequence]] | ||
Latest revision as of 03:45, 19 December 2007
Construction details
pME-nlsEGFP was made by PCR amplification of an nls-EGFP (EGFP with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
Sequence
Annotated sequence, Genbank format:
pME-nlsEGFP Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
pME-nlsEGFP sequence
Crude map
Screenshot from Sequencher showing locations of components:
