From Tol2Kit
Revision as of 01:48, 24 March 2007 by Chi-bin (talk | contribs) (New page: === Construction details === pME-nlsEGFP was made by PCR amplification of an nls-EGFP (nuclear-localized GFP) cassette, using primers that add att sites, followed by a BP reaction. Sequen...)
Construction details
pME-nlsEGFP was made by PCR amplification of an nls-EGFP (nuclear-localized GFP) cassette, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
pME-nlsEGFP sequence
Crude map
Screenshot from Sequencher showing locations of components:
