From Tol2Kit
Construction details
pME-nlsEGFP was made by PCR amplification of an nls-EGFP (EGFP with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
pME-nlsEGFP sequence
Crude map
Screenshot from Sequencher showing locations of components:
