From Tol2Kit
(New page: === Construction details === pME-nlsmCherry was made by PCR amplification of an nls-mCherry (mCherry with nuclear-localization signal) cassette, using primers that add att sites, followed ...) |
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=== Construction details === | === Construction details === | ||
| − | pME-nlsmCherry was made by PCR amplification of an nls-mCherry (mCherry with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction. | + | pME-nlsmCherry was made by PCR amplification of an nls-mCherry (mCherry with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction. |
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| + | Sequencing finds a single PCR error in the insert, encoding an H30R mutation in mCherry. However, expression constructs made with this cassette show appropriate function (nuclear-localized fluorescence). | ||
=== Crudely annotated sequence === | === Crudely annotated sequence === | ||
Revision as of 02:04, 24 March 2007
Construction details
pME-nlsmCherry was made by PCR amplification of an nls-mCherry (mCherry with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction.
Sequencing finds a single PCR error in the insert, encoding an H30R mutation in mCherry. However, expression constructs made with this cassette show appropriate function (nuclear-localized fluorescence).
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
pME-nlsmCherry sequence
Crude map
Screenshot from Sequencher showing locations of components:
