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Sequencing finds a single PCR error in the insert, encoding an H30R mutation in mCherry. However, expression constructs made with this cassette show appropriate function (nuclear-localized fluorescence).
 
Sequencing finds a single PCR error in the insert, encoding an H30R mutation in mCherry. However, expression constructs made with this cassette show appropriate function (nuclear-localized fluorescence).
  
=== Crudely annotated sequence ===
+
=== Sequence ===
 +
Annotated sequence, Genbank format:<br>
 +
[[pME-nlsmCherry Genbank]]
 +
 
 
FASTA file with the full-length sequence as well as sequences of individual components:<br>
 
FASTA file with the full-length sequence as well as sequences of individual components:<br>
 
[[pME-nlsmCherry sequence]]
 
[[pME-nlsmCherry sequence]]

Latest revision as of 03:46, 19 December 2007

Construction details

pME-nlsmCherry was made by PCR amplification of an nls-mCherry (mCherry with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction.

Sequencing finds a single PCR error in the insert, encoding an H30R mutation in mCherry. However, expression constructs made with this cassette show appropriate function (nuclear-localized fluorescence).

Sequence

Annotated sequence, Genbank format:
pME-nlsmCherry Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
pME-nlsmCherry sequence

Crude map

Screenshot from Sequencher showing locations of components:
PME-nlsmCherry.png