From Tol2Kit
(New page: === Construction details === pME-nlsmCherry was made by PCR amplification of an nls-mCherry (mCherry with nuclear-localization signal) cassette, using primers that add att sites, followed ...) |
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=== Construction details === | === Construction details === | ||
| − | pME-nlsmCherry was made by PCR amplification of an nls-mCherry (mCherry with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction. | + | pME-nlsmCherry was made by PCR amplification of an nls-mCherry (mCherry with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction. |
| + | |||
| + | Sequencing finds a single PCR error in the insert, encoding an H30R mutation in mCherry. However, expression constructs made with this cassette show appropriate function (nuclear-localized fluorescence). | ||
| + | |||
| + | === Sequence === | ||
| + | Annotated sequence, Genbank format:<br> | ||
| + | [[pME-nlsmCherry Genbank]] | ||
| − | |||
FASTA file with the full-length sequence as well as sequences of individual components:<br> | FASTA file with the full-length sequence as well as sequences of individual components:<br> | ||
[[pME-nlsmCherry sequence]] | [[pME-nlsmCherry sequence]] | ||
Latest revision as of 03:46, 19 December 2007
Construction details
pME-nlsmCherry was made by PCR amplification of an nls-mCherry (mCherry with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction.
Sequencing finds a single PCR error in the insert, encoding an H30R mutation in mCherry. However, expression constructs made with this cassette show appropriate function (nuclear-localized fluorescence).
Sequence
Annotated sequence, Genbank format:
pME-nlsmCherry Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
pME-nlsmCherry sequence
Crude map
Screenshot from Sequencher showing locations of components:
