From Tol2Kit

Jump to: navigation, search

[edit] Construction details

pME-EGFPCAAX was made by PCR amplification of EGFP-CAAX, using primers to add att sites, followed by a BP reaction. EGFP-CAAX is EGFP with a C-terminal fusion of 21 amino acids of human Harvey Ras, encoding a prenylation signal. Sequencing confirms that all bases of the insert are correct.

[edit] Sequence

Annotated sequence, Genbank format:

FASTA file with the full-length sequence as well as sequences of individual components:
pME-EGFPCAAX sequence

[edit] Crude map

Screenshot from Sequencher showing locations of components:

Personal tools