PME-EGFPCAAX

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[edit] Construction details

pME-EGFPCAAX was made by PCR amplification of EGFP-CAAX, using primers to add att sites, followed by a BP reaction. EGFP-CAAX is EGFP with a C-terminal fusion of 21 amino acids of human Harvey Ras, encoding a prenylation signal. Sequencing confirms that all bases of the insert are correct.

[edit] Sequence

Annotated sequence, Genbank format:
pME-EGFPCAAX Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
pME-EGFPCAAX sequence

[edit] Crude map

Screenshot from Sequencher showing locations of components:
Image:pME-EGFPCAAX.png

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