(New page: === Construction details === p5E-''h2afx'' was made by PCR amplification of 1 kb genomic DNA immediately upstream of the start codon of ''h2afx'' (NM_201073), using primers that added att ...) |
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Expression data for this gene from the Thisses (see [http://zfin.org/cgi-bin/webdriver?MIval=aa-xpatselect.apg&query_results=exist&START=0&searchtype=contains&gene_name=h2afx ZFIN]) that shows that ''h2afx'' is expressed throughout the embryo in presumptive proliferative regions. | Expression data for this gene from the Thisses (see [http://zfin.org/cgi-bin/webdriver?MIval=aa-xpatselect.apg&query_results=exist&START=0&searchtype=contains&gene_name=h2afx ZFIN]) that shows that ''h2afx'' is expressed throughout the embryo in presumptive proliferative regions. | ||
| − | === | + | === Sequence === |
| + | Annotated sequence, Genbank format:<br> | ||
| + | [[p5E-h2afx Genbank]] | ||
| + | |||
FASTA file with the full-length sequence as well as sequences of individual components:<br> | FASTA file with the full-length sequence as well as sequences of individual components:<br> | ||
[[p5E-h2afx sequence]] | [[p5E-h2afx sequence]] | ||
Latest revision as of 03:41, 19 December 2007
Construction details
p5E-h2afx was made by PCR amplification of 1 kb genomic DNA immediately upstream of the start codon of h2afx (NM_201073), using primers that added att sites, followed by a BP reaction. This genomic element was originally isolated by John Parant in Joe Yost's lab. Sequencing confirms that the insert is correct, although there are numerous single-base changes compared to the Sanger genomic sequence (presumably representing SNPs).
Expression data for this gene from the Thisses (see ZFIN) that shows that h2afx is expressed throughout the embryo in presumptive proliferative regions.
Sequence
Annotated sequence, Genbank format:
p5E-h2afx Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
p5E-h2afx sequence
Crude map
Screenshot from Sequencher showing locations of components:
