From Tol2Kit
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Sequencing shows a single base mutation in hsp70 (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock. | Sequencing shows a single base mutation in hsp70 (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock. | ||
| − | === | + | === Sequence === |
| + | Annotated sequence, Genbank format:<br> | ||
| + | [[p5E-hsp70 Genbank]] | ||
| + | |||
FASTA file with the full-length sequence as well as sequences of individual components:<br> | FASTA file with the full-length sequence as well as sequences of individual components:<br> | ||
[[p5E-hsp70 sequence]] | [[p5E-hsp70 sequence]] | ||
Revision as of 03:42, 19 December 2007
Construction details
p5E-hsp70 was made by PCR amplification of the 1.5 kb hsp70 promoter (Genbank AF158020), using primers to add att sites, followed by a BP reaction. Sequencing shows a single base mutation in hsp70 (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock.
Sequence
Annotated sequence, Genbank format:
p5E-hsp70 Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
p5E-hsp70 sequence
Crude map
Screenshot from Sequencher showing locations of components:
