From Tol2Kit
(New page: === Construction details === pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR) to the N terminus of mCherry, using primers that add att sites, followed by a...) |
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=== Construction details === | === Construction details === | ||
| − | pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR) to the N terminus of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, A883G. | + | pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR; official ZFIN gene name ''h2afv'') to the N terminus of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, A883G. |
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| + | === Sequence === | ||
| + | Annotated sequence, Genbank format:<br> | ||
| + | [[pME-H2AmCherry Genbank]] | ||
| − | |||
FASTA file with the full-length sequence as well as sequences of individual components:<br> | FASTA file with the full-length sequence as well as sequences of individual components:<br> | ||
[[pME-H2AmCherry sequence]] | [[pME-H2AmCherry sequence]] | ||
Latest revision as of 11:55, 1 April 2008
Construction details
pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR; official ZFIN gene name h2afv) to the N terminus of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, A883G.
Sequence
Annotated sequence, Genbank format:
pME-H2AmCherry Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
pME-H2AmCherry sequence
Crude map
Screenshot from Sequencher showing locations of components:
