From Tol2Kit
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Here is a list of the constructs in the Tol2kit. | Here is a list of the constructs in the Tol2kit. | ||
| − | + | We have included crudely annotated sequences and maps for several constructs, 2/7/07. | |
| + | Better-formatted information for all sequences is planned Real Soon Now. | ||
'''Constructs in the Tol2kit v1 (Feb 2007)''' | '''Constructs in the Tol2kit v1 (Feb 2007)''' | ||
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!384||pME-EGFPCAAX | !384||pME-EGFPCAAX | ||
| − | |membrane-localized ( | + | |membrane-localized (prenylated) EGFP; fused to 21 aa of H-ras||KMK |
|- | |- | ||
!385||pME-nlsEGFP | !385||pME-nlsEGFP | ||
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|mCherry for C-terminal fusions, plus SV40 late polyA||MEH | |mCherry for C-terminal fusions, plus SV40 late polyA||MEH | ||
|- | |- | ||
| − | !389||p3E-IRES-EGFPpA | + | !389||[[p3E-IRES-EGFPpA]]] |
|IRES driving EGFP plus SV40 late polyA||KMK | |IRES driving EGFP plus SV40 late polyA||KMK | ||
|- | |- | ||
| − | !390||p3E-IRES-EGFPCAAXpA | + | !390||[[p3E-IRES-EGFPCAAXpA]] |
|IRES driving prenylated EGFP plus SV40 late polyA||KMK | |IRES driving prenylated EGFP plus SV40 late polyA||KMK | ||
|- | |- | ||
| − | !391||p3E-IRES-nlsEGFPpA | + | !391||[[p3E-IRES-nlsEGFPpA]] |
|IRES driving nuclear EGFP plus SV40 late polyA||KMK | |IRES driving nuclear EGFP plus SV40 late polyA||KMK | ||
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|pDestTol2pA with cmlc2:egfp transgenesis marker||EF | |pDestTol2pA with cmlc2:egfp transgenesis marker||EF | ||
|- | |- | ||
| − | !394||pDestTol2pA2 | + | !394||[[pDestTol2pA2]] |
|pDestTol2pA with ~2 kb extraneous sequence removed||EF | |pDestTol2pA with ~2 kb extraneous sequence removed||EF | ||
|- | |- | ||
| − | !395||pDestTol2CG2 | + | !395||[[pDestTol2CG2]] |
|pDestTol2CG2 with ~2 kb extraneous sequence removed||EF | |pDestTol2CG2 with ~2 kb extraneous sequence removed||EF | ||
Revision as of 19:08, 7 February 2007
Here is a list of the constructs in the Tol2kit. We have included crudely annotated sequences and maps for several constructs, 2/7/07. Better-formatted information for all sequences is planned Real Soon Now.
Constructs in the Tol2kit v1 (Feb 2007)
| # | name | insert | made by |
|---|---|---|---|
| 5' entry clones, attL4-R1 (kan resistant) | |||
| 299 | p5E-bactin2 | 5.3 kb beta-actin promoter (ubiquitous) | KMK |
| 380 | p5E-h2afx | 1 kb histone2A-X promoter (quasi-ubiquitous) | KMK |
| 382 | p5E-CMV/SP6 | 1 kb CMV/SP6 cassette from pCS2+ | KMK |
| 222 | p5E-hsp70 | 1.5 kb hsp70 promoter for heat-shock induction | BM |
| 327 | p5E-UASE1B | 10x UAS element and basal promoter for Gal4 response | DSC |
| 228 | p5E-MCS | multi-cloning site from pBluescript | BM |
| 381 | p5E-Fse-Asc | restriction sites for 8-cutters FseI and AscI | EF |
| middle entry clones, attL1-L2 (kan resistant) | |||
| 383 | pME-EGFP | EGFP | KMK |
| 384 | pME-EGFPCAAX | membrane-localized (prenylated) EGFP; fused to 21 aa of H-ras | KMK |
| 385 | pME-nlsEGFP | nuclear-localized EGFP | KMK |
| 386 | pME-mCherry | monomeric red fluorophore mCherry | KMK |
| 232 | pME-mCherryCAAX | prenylated mCherry | KMK |
| 233 | pME-nlsmCherry | nuclear-localized mCherry | KMK |
| 234 | pME-H2AmCherry | mCherry fused to zebrafish histone H2A.F/Z | KMK |
| 387 | pME-Gal4VP16 | Gal4 DNA binding domain fused to VP16 transactivation domain | KMK |
| 3' entry clones, attR2-L3 (kan resistant) | |||
| 302 | p3E-polyA | SV40 late polyA signal | KMK |
| 229 | p3E-MTpA | 6x myc tag for C-terminal fusions, plus SV40 late polyA | BM |
| 366 | p3E-EGFPpA | EGFP for C-terminal fusions, plus SV40 late polyA | MEH |
| 388 | p3E-mCherrypA | mCherry for C-terminal fusions, plus SV40 late polyA | MEH |
| 389 | p3E-IRES-EGFPpA] | IRES driving EGFP plus SV40 late polyA | KMK |
| 390 | p3E-IRES-EGFPCAAXpA | IRES driving prenylated EGFP plus SV40 late polyA | KMK |
| 391 | p3E-IRES-nlsEGFPpA | IRES driving nuclear EGFP plus SV40 late polyA | KMK |
| destination vectors, attR4-R3 (amp/chlor resistant; grow in ccdB-tolerant cells) | |||
| 392 | pDestTol2pA* | attR4-R3 gate with SV40 polyA flanked by Tol2 inverted repeats | CG |
| 393 | pDestTol2CG* | pDestTol2pA with cmlc2:egfp transgenesis marker | EF |
| 394 | pDestTol2pA2 | pDestTol2pA with ~2 kb extraneous sequence removed | EF |
| 395 | pDestTol2CG2 | pDestTol2CG2 with ~2 kb extraneous sequence removed | EF |
| transposase clones | |||
| 396 | pCS2FA-transposase | for in vitro transcription of capped Tol2 transposase mRNA | KMK |
Notes
- # is the Chien lab stock number
- made by shows who made the construct:
- CG: Clemens Grabher, Look lab
- BM: Ben Mangum, Chien lab
- DSC, Doug Campbell, Chien lab
- EF, Esther Fujimoto, Chien lab
- KMK, Kristen Kwan, Chien lab
- MEH, Melissa Hardy, Chien lab
- pDestTol2pA and pDestTol2CG have been superseded by pDestTol2pA2 and pDestTol2CG2, and are no longer being distributed.