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(Construction details)
 
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=== Construction details ===
 
=== Construction details ===
pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR) to the N terminus of mCherry, using primers that add att sites, followed by a BP reaction.  Sequencing finds a single silent mutation, A883G.
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pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR; official ZFIN gene name ''h2afv'') to the N terminus of mCherry, using primers that add att sites, followed by a BP reaction.  Sequencing finds a single silent mutation, A883G.
  
 
=== Sequence ===
 
=== Sequence ===

Latest revision as of 11:55, 1 April 2008

Construction details

pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR; official ZFIN gene name h2afv) to the N terminus of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, A883G.

Sequence

Annotated sequence, Genbank format:
pME-H2AmCherry Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
pME-H2AmCherry sequence

Crude map

Screenshot from Sequencher showing locations of components:
PME-H2AmCherry.png