Difference between revisions of "P5E-hsp70l"

Revision as of 11:57, 1 April 2008

Construction details

p5E-hsp70 was made by PCR amplification of the 1.5 kb hsp70 promoter (Genbank AF158020), using primers to add att sites, followed by a BP reaction. Sequencing shows a single base mutation in hsp70 (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock.

Sequence

Annotated sequence, Genbank format:
p5E-hsp70 Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
p5E-hsp70 sequence

Crude map

Screenshot from Sequencher showing locations of components: