From Tol2Kit
Revision as of 11:57, 1 April 2008 by Chi-bin (talk | contribs) (P5E-hsp70 moved to P5E-hsp70l: Changing name to match official ZFIN gene name)
Construction details
p5E-hsp70 was made by PCR amplification of the 1.5 kb hsp70 promoter (Genbank AF158020), using primers to add att sites, followed by a BP reaction. Sequencing shows a single base mutation in hsp70 (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock.
Sequence
Annotated sequence, Genbank format:
p5E-hsp70 Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
p5E-hsp70 sequence
Crude map
Screenshot from Sequencher showing locations of components:
