From Tol2Kit
(New page: === Construction details === pME-EGFPCAAX was made by PCR amplification of EGFP-CAAX, using primers to add att sites, followed by a BP reaction. EGFP-CAAX is EGFP with a C-terminal fusion ...) |
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pME-EGFPCAAX was made by PCR amplification of EGFP-CAAX, using primers to add att sites, followed by a BP reaction. EGFP-CAAX is EGFP with a C-terminal fusion of 21 amino acids of human Harvey Ras, encoding a prenylation signal. Sequencing confirms that all bases of the insert are correct. | pME-EGFPCAAX was made by PCR amplification of EGFP-CAAX, using primers to add att sites, followed by a BP reaction. EGFP-CAAX is EGFP with a C-terminal fusion of 21 amino acids of human Harvey Ras, encoding a prenylation signal. Sequencing confirms that all bases of the insert are correct. | ||
| − | === | + | === Sequence === |
| + | Annotated sequence, Genbank format:<br> | ||
| + | [[pME-EGFPCAAX Genbank]] | ||
| + | |||
FASTA file with the full-length sequence as well as sequences of individual components:<br> | FASTA file with the full-length sequence as well as sequences of individual components:<br> | ||
[[pME-EGFPCAAX sequence]] | [[pME-EGFPCAAX sequence]] | ||
Latest revision as of 03:44, 19 December 2007
Construction details
pME-EGFPCAAX was made by PCR amplification of EGFP-CAAX, using primers to add att sites, followed by a BP reaction. EGFP-CAAX is EGFP with a C-terminal fusion of 21 amino acids of human Harvey Ras, encoding a prenylation signal. Sequencing confirms that all bases of the insert are correct.
Sequence
Annotated sequence, Genbank format:
pME-EGFPCAAX Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
pME-EGFPCAAX sequence
Crude map
Screenshot from Sequencher showing locations of components:
