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(New page: === Construction details === pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR) to the N terminus of mCherry, using primers that add att sites, followed by a...)
 
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pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR) to the N terminus of mCherry, using primers that add att sites, followed by a BP reaction.  Sequencing finds a single silent mutation, A883G.
 
pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR) to the N terminus of mCherry, using primers that add att sites, followed by a BP reaction.  Sequencing finds a single silent mutation, A883G.
  
=== Crudely annotated sequence ===
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=== Sequence ===
 +
Annotated sequence, Genbank format:<br>
 +
[[pME-H2AmCherry Genbank]]
 +
 
 
FASTA file with the full-length sequence as well as sequences of individual components:<br>
 
FASTA file with the full-length sequence as well as sequences of individual components:<br>
 
[[pME-H2AmCherry sequence]]
 
[[pME-H2AmCherry sequence]]

Revision as of 03:46, 19 December 2007

Construction details

pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR) to the N terminus of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, A883G.

Sequence

Annotated sequence, Genbank format:
pME-H2AmCherry Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
pME-H2AmCherry sequence

Crude map

Screenshot from Sequencher showing locations of components:
PME-H2AmCherry.png