From Tol2Kit
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- 21:35, 18 April 2007 (diff | hist) . . (+37) . . Main Page (→About this site)
- 05:07, 18 April 2007 (diff | hist) . . (+56) . . N File:PDestTol2CG.png (Sequencher screenshot showing components of pDestTol2CG.) (current)
- 05:06, 18 April 2007 (diff | hist) . . (+21,422) . . N PDestTol2CG sequence (New page: <pre> >pDestTol2CG CACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAA TACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGC CCTTATTCCCTTTTTT...)
- 05:05, 18 April 2007 (diff | hist) . . (+604) . . N PDestTol2CG (New page: === Construction details === pDestTol2CG was made by PCRing the cmlc2:egfp-pA cassette, adding BglII sites in the primers, then cloning into the unique BglII site of pDestTol2pA and select...)
- 05:01, 18 April 2007 (diff | hist) . . (+18,833) . . PDestTol2pA sequence
- 04:56, 18 April 2007 (diff | hist) . . (+56) . . N File:PDestTol2pA.png (Sequencher screenshot showing components of pDestTol2pA.) (current)
- 04:54, 18 April 2007 (diff | hist) . . (+395) . . N PDestTol2pA (New page: === Construction details === pDestTol2pA was built by Clemens Grabher; we are in the process of asking him for construction details. === Crudely annotated sequence === FASTA file with the...)
- 04:53, 18 April 2007 (diff | hist) . . (+148) . . List of entry and destination vectors
- 02:51, 30 March 2007 (diff | hist) . . (+2) . . m Main Page (→Clone distribution)
- 03:34, 24 March 2007 (diff | hist) . . (+9) . . m PCS2FA-transposase (→Construction details)
- 03:33, 24 March 2007 (diff | hist) . . (+63) . . N File:PCS2FA-transposase.png (Sequencher screenshot showing components of pCS2FA-transposase.) (current)
- 03:32, 24 March 2007 (diff | hist) . . (+11,476) . . N PCS2FA-transposase sequence (New page: <pre> >pCS2FA-transposase TCGACCATAGCCAATTCAATATGGCGTATATGGACTCATGCCAATTCAAT ATGGTGGATCTGGACCTGTGCCAATTCAATATGGCGTATATGGACTCGTG CCAATTCAATATGGTGGATCTGGACCCCAGCCAATTCAATATGGCGGACT TGGCACCAT...) (current)
- 03:31, 24 March 2007 (diff | hist) . . (+748) . . N PCS2FA-transposase (New page: === Construction details === pCS2FA-transposase was made by PCRing the Tol2 transpoase cds from the original Kawakami clone, adding restriction sites, then conventionally subcloned into pC...)
- 03:26, 24 March 2007 (diff | hist) . . (-10) . . List of entry and destination vectors
- 03:24, 24 March 2007 (diff | hist) . . (+99) . . List of entry and destination vectors
- 03:12, 24 March 2007 (diff | hist) . . (+58) . . N File:P3E-mCherrypA.png (Sequencher screenshot showing components of p3E-mCherrypA.) (current)
- 03:11, 24 March 2007 (diff | hist) . . (+7,642) . . N P3E-mCherrypA sequence (New page: <pre> >p3E-mCherrypA CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTC...) (current)
- 03:11, 24 March 2007 (diff | hist) . . (+672) . . N P3E-mCherrypA (New page: === Construction details === p3E-mCherrypA was made by PCR amplification of mCherry and an SV40 late polyadenylation signal from a pCS2+-based construct, using primers to add att sites, fo...)
- 03:07, 24 March 2007 (diff | hist) . . (+55) . . N File:P3E-EGFPpA.png (Sequencher screenshot showing components of p3E-EGFPpA.) (current)
- 03:07, 24 March 2007 (diff | hist) . . (+32) . . P3E-EGFPpA (→Construction details)
- 03:06, 24 March 2007 (diff | hist) . . (+7,836) . . N P3E-EGFPpA sequence (New page: <pre> >p3E_EGFPpA CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATT...) (current)
- 03:05, 24 March 2007 (diff | hist) . . (+714) . . N P3E-EGFPpA (New page: === Construction details === p3E-EGFPpA was made by PCR amplification of EGFP and an SV40 late polyadenylation signal from a pCS2+-based plasmid, using primers to add att sites, followed b...)
- 03:03, 24 March 2007 (diff | hist) . . (+5) . . m P3E-MTpA (→Construction details)
- 03:02, 24 March 2007 (diff | hist) . . (+53) . . N File:P3E-MTpA.png (Sequencher screenshot showing components of p3E-MTpA.) (current)
- 03:02, 24 March 2007 (diff | hist) . . (0) . . m P3E-MTpA (→Construction details)
- 03:01, 24 March 2007 (diff | hist) . . (+6,300) . . N P3E-MTpA sequence (New page: <pre> >p3E_MT-pA CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTA...) (current)
- 03:00, 24 March 2007 (diff | hist) . . (+507) . . N P3E-MTpA (New page: === Construction details === p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 polyadenylation signal from pCS2-MT, using primers to add att sequences, followed by a BP reaction....)
- 02:35, 24 March 2007 (diff | hist) . . (0) . . m P3E-polyA (→Construction details)
- 02:34, 24 March 2007 (diff | hist) . . (+54) . . N File:P3E-polyA.png (Sequencher screenshot showing components of p3E-polyA.) (current)
- 02:32, 24 March 2007 (diff | hist) . . (+6) . . P3E-polyA sequence (current)
- 02:32, 24 March 2007 (diff | hist) . . (+5,363) . . N P3E-polyA sequence (New page: <pre> >p3E-polyA CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTA...)
- 02:31, 24 March 2007 (diff | hist) . . (+501) . . N P3E-polyA (New page: === Construction details === p3E-polyA was made by PCR amplificaiton of the SV40 late polyadenylation signal from pCS2+, using primers that add att sites, followed by a BP reaction. Sequen...)
- 02:18, 24 March 2007 (diff | hist) . . (+57) . . N File:PME-Gal4VP16.png (Sequencher screenshot showing components of pME-Gal4VP16.) (current)
- 02:18, 24 March 2007 (diff | hist) . . (+63) . . m PME-Gal4VP16 (→Construction details)
- 02:16, 24 March 2007 (diff | hist) . . (+6,586) . . N PME-Gal4VP16 sequence (New page: <pre> >pME_Gal4VP16 CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCA...) (current)
- 02:16, 24 March 2007 (diff | hist) . . (+460) . . N PME-Gal4VP16 (New page: === Construction details === pME-Gal4VP16 was made by PCR amplification of Gal4VP16, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation,...)
- 02:14, 24 March 2007 (diff | hist) . . (+59) . . N File:PME-H2AmCherry.png (Sequencher screenshot showing components of pME-H2AmCherry.) (current)
- 02:13, 24 March 2007 (diff | hist) . . (+7,973) . . N PME-H2AmCherry sequence (New page: <pre> >pME-H2AF/Z-mCherry CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCC...) (current)
- 02:13, 24 March 2007 (diff | hist) . . (+520) . . N PME-H2AmCherry (New page: === Construction details === pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR) to the N terminus of mCherry, using primers that add att sites, followed by a...)
- 02:06, 24 March 2007 (diff | hist) . . (+59) . . N File:PME-nlsmCherry.png (Sequencher screenshot showing components of pME-nlsmCherry.) (current)
- 02:06, 24 March 2007 (diff | hist) . . (+6,864) . . N PME-nlsmCherry sequence (New page: <pre> >pME-nlsmCherry CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATT...) (current)
- 02:04, 24 March 2007 (diff | hist) . . (+142) . . PME-nlsmCherry
- 02:02, 24 March 2007 (diff | hist) . . (+536) . . N PME-nlsmCherry (New page: === Construction details === pME-nlsmCherry was made by PCR amplification of an nls-mCherry (mCherry with nuclear-localization signal) cassette, using primers that add att sites, followed ...)
- 01:59, 24 March 2007 (diff | hist) . . (+60) . . N File:PME-mCherryCAAX.png (Sequencher screenshot showing components of pME-mCherryCAAX.) (current)
- 01:58, 24 March 2007 (diff | hist) . . (+7,001) . . N PME-mCherryCAAX H80D sequence (New page: <pre> >pME-mCherryCAAX CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGAT...)
- 01:57, 24 March 2007 (diff | hist) . . (+601) . . N PME-mCherryCAAX H80D (New page: === Construction details === pME-mCherryCAAX was made by PCR amplification of mCherry-CAAX, using primers to add att sites, followed by a BP reaction. mCherry-CAAX is mCherry with a C-term...)
- 01:54, 24 March 2007 (diff | hist) . . (+56) . . N File:PME-mCherry.png (Sequencher screenshot showing components of pME-mCherry.) (current)
- 01:53, 24 March 2007 (diff | hist) . . (+6,771) . . N PME-mCherry sequence (New page: <pre> >pME-mCherry CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCAT...) (current)
- 01:52, 24 March 2007 (diff | hist) . . (+468) . . N PME-mCherry (New page: === Construction details === pME-mCherry was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the i...)
- 01:51, 24 March 2007 (diff | hist) . . (+16) . . m PME-nlsEGFP (→Construction details)
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