From Tol2Kit
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- 01:50, 24 March 2007 (diff | hist) . . (+56) . . N File:PME-nlsEGFP.png (Sequencher screenshot showing components of pME-nlsEGFP.) (current)
- 01:49, 24 March 2007 (diff | hist) . . (+6,970) . . N PME-nlsEGFP sequence (New page: <pre> >pME-nlsEGFP CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCAT...) (current)
- 01:48, 24 March 2007 (diff | hist) . . (+505) . . N PME-nlsEGFP (New page: === Construction details === pME-nlsEGFP was made by PCR amplification of an nls-EGFP (nuclear-localized GFP) cassette, using primers that add att sites, followed by a BP reaction. Sequen...)
- 01:46, 24 March 2007 (diff | hist) . . (+57) . . N File:PME-EGFPCAAX.png (Sequencher screenshot showing components of pME-EGFPCAAX.) (current)
- 01:45, 24 March 2007 (diff | hist) . . (+7,079) . . N PME-EGFPCAAX sequence (New page: <pre> >pME-EGFP-CAAX CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTC...) (current)
- 01:44, 24 March 2007 (diff | hist) . . (+583) . . N PME-EGFPCAAX (New page: === Construction details === pME-EGFPCAAX was made by PCR amplification of EGFP-CAAX, using primers to add att sites, followed by a BP reaction. EGFP-CAAX is EGFP with a C-terminal fusion ...)
- 01:40, 24 March 2007 (diff | hist) . . (+37) . . m PME-EGFP (→Construction details)
- 01:39, 24 March 2007 (diff | hist) . . (+1) . . PME-EGFP (→Construction details)
- 01:38, 24 March 2007 (diff | hist) . . (+186) . . PME-EGFP (→Construction details)
- 01:35, 24 March 2007 (diff | hist) . . (+53) . . N File:PME-EGFP.png (Sequencher screenshot showing components of pME-EGFP.) (current)
- 01:34, 24 March 2007 (diff | hist) . . (+7,002) . . N PME-EGFP sequence (New page: <pre> >pME-EGFP CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTAA...) (current)
- 01:32, 24 March 2007 (diff | hist) . . (+456) . . N PME-EGFP (New page: === Construction details === pME-EGFP was made by PCR amplification of EGFP, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert ...)
- 01:27, 24 March 2007 (diff | hist) . . (+52) . . List of entry and destination vectors
- 03:53, 23 March 2007 (diff | hist) . . (+41) . . P5E-UAS (→Construction details)
- 03:52, 23 March 2007 (diff | hist) . . (+52) . . N File:P5E-UAS.png (Sequencher screenshot showing components of p5E-UAS.) (current)
- 03:51, 23 March 2007 (diff | hist) . . (+6,169) . . N P5E-UAS sequence (New page: <pre> >p5E_UAS CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTAAT...)
- 03:51, 23 March 2007 (diff | hist) . . (+997) . . N P5E-UAS (New page: === Construction details === p5E-UAS was made by conventionally subcloning into p5E-MCS a HindIII-EcoRI fragment from a UAS-E1b construct from Reinhard Köster, containing multimerized Gal...)
- 03:33, 23 March 2007 (diff | hist) . . (+19) . . m P5E-hsp70l (→Construction details)
- 03:32, 23 March 2007 (diff | hist) . . (+58) . . N File:P5E-hsp70.png (Sequencher screenshot showing components of p5E-''hsp70''.) (current)
- 03:31, 23 March 2007 (diff | hist) . . (+9,424) . . N P5E-hsp70l sequence (New page: <pre> >p5E-hsp70 CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTA...)
- 03:31, 23 March 2007 (diff | hist) . . (+597) . . N P5E-hsp70l (New page: === Construction details === p5E-''hsp70'' was made by PCR amplification of the 1.5 kb hsp70 promoter, using primers to add att sites, followed by a BP reaction. Sequencing shows a single...)
- 03:24, 23 March 2007 (diff | hist) . . (+56) . . N File:P5E-CMV-SP6.png (Sequencher screenshot showing components of p5E-CMV/SP6.) (current)
- 03:23, 23 March 2007 (diff | hist) . . (+7,957) . . N P5E-CMV-SP6 sequence (New page: <pre> >p5E-CMV-SP6 CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCAT...) (current)
- 03:22, 23 March 2007 (diff | hist) . . (+821) . . N P5E-CMV/SP6 (New page: === Construction details === p5E-CMV/SP6 was made by PCR amplification of the sCMV and SP6 promoters from pCS2+, using primers that added att sites, followed by a BP reaction. This 5' el...)
- 22:29, 22 March 2007 (diff | hist) . . (+58) . . N File:P5E-h2afx.png (Sequencher screenshot showing components of p5E-''h2afx''.) (current)
- 22:28, 22 March 2007 (diff | hist) . . (+7,753) . . N P5E-h2afx sequence (New page: <pre> >p5E-h2afx CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTA...) (current)
- 22:27, 22 March 2007 (diff | hist) . . (+1,008) . . N P5E-h2afx (New page: === Construction details === p5E-''h2afx'' was made by PCR amplification of 1 kb genomic DNA immediately upstream of the start codon of ''h2afx'' (NM_201073), using primers that added att ...)
- 22:09, 22 March 2007 (diff | hist) . . (+36) . . List of entry and destination vectors
- 21:35, 22 March 2007 (diff | hist) . . (+822) . . Main Page
- 23:20, 19 March 2007 (diff | hist) . . (+371) . . Protocols
- 23:16, 19 March 2007 (diff | hist) . . (+82) . . List of entry and destination vectors
- 23:14, 19 March 2007 (diff | hist) . . (+152) . . P5E-MCS sequence (current)
- 23:13, 19 March 2007 (diff | hist) . . (-2,735) . . P5E-Fse-Asc sequence (current)
- 23:12, 19 March 2007 (diff | hist) . . (-2,731) . . P3E-IRES-EGFPpA sequence (current)
- 23:11, 19 March 2007 (diff | hist) . . (-3,565) . . P3E-IRES-EGFPCAAXpA sequence (current)
- 23:10, 19 March 2007 (diff | hist) . . (-2,738) . . P3E-IRES-nlsEGFPpA sequence (current)
- 23:08, 19 March 2007 (diff | hist) . . (-1,362) . . PDestTol2pA2 sequence
- 23:05, 19 March 2007 (diff | hist) . . (-1,393) . . PDestTol2CG2 sequence
- 23:00, 19 March 2007 (diff | hist) . . (-6) . . m PDestTol2CG2
- 22:59, 19 March 2007 (diff | hist) . . (-6) . . m PDestTol2pA2
- 22:58, 19 March 2007 (diff | hist) . . (-6) . . m P3E-IRES-nlsEGFPpA
- 22:57, 19 March 2007 (diff | hist) . . (-6) . . m P3E-IRES-EGFPCAAXpA
- 22:57, 19 March 2007 (diff | hist) . . (-6) . . m P3E-IRES-EGFPpA
- 22:56, 19 March 2007 (diff | hist) . . (-6) . . m P5E-Fse-Asc
- 22:55, 19 March 2007 (diff | hist) . . (-6) . . P5E-MCS
- 22:53, 19 March 2007 (diff | hist) . . (+56) . . N File:P5E-bactin2.png (Sequencher screenshot showing components of p5E-bactin2.) (current)
- 22:52, 19 March 2007 (diff | hist) . . (+22,557) . . N P5E-bactin2 sequence (New page: <pre> >p5E-bactin2 CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCAT...) (current)
- 21:00, 19 March 2007 (diff | hist) . . (+1,098) . . N P5E-bactin2 (New page: === Construction details === ''bactin2'' genomic sequence was derived from a 5.3 kb clone (gift of Ken Poss), which was derived in turn from a 10 kb upstream construct from Shin-ichi Higas...)
- 20:51, 19 March 2007 (diff | hist) . . (+16) . . List of entry and destination vectors
- 14:34, 24 February 2007 (diff | hist) . . (+3,536) . . N Att site sequences (New page: == att site shared sequences == Here is a set of "shared" sequences--the core stretch that is shared between attB/P/L/R for att1-4. These are useful for stitching together entry and destin...)
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