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|+  
 
|+  
−
! # !! name !! insert !! size (bp) !! made by
+
! # !! name !! insert !! size (bp) !! made by !! ver !! MTA?
  
 
|-
 
|-
−
! colspan="5" style="background:#ffdead;" |5' entry clones, attL4-R1 (kan resistant)
+
! colspan="7" style="background:#ffdead;" |5' entry clones, attL4-R1 (kan resistant)
 
|-
 
|-
 
!299||[[p5E-bactin2|p5E-''bactin2'']]
 
!299||[[p5E-bactin2|p5E-''bactin2'']]
−
|5.3 kb beta-actin promoter (ubiquitous)||7950||KMK
+
|5.3 kb beta-actin promoter (ubiquitous)||7950||KMK||1.0||
 
|-
 
|-
 
!380||[[p5E-h2afx|p5E-''h2afx'']]
 
!380||[[p5E-h2afx|p5E-''h2afx'']]
−
|1 kb histone2A-X promoter (quasi-ubiquitous)||3604||KMK
+
|1 kb histone2A-X promoter (quasi-ubiquitous)||3604||KMK||1.0||
 
|-
 
|-
 
!382||[[p5E-CMV/SP6]]
 
!382||[[p5E-CMV/SP6]]
−
|1 kb CMV/SP6 cassette from pCS2+||3704||KMK
+
|1 kb CMV/SP6 cassette from pCS2+||3704||KMK||1.0||
 
|-
 
|-
 
!222||[[p5E-hsp70|p5E-''hsp70'']]
 
!222||[[p5E-hsp70|p5E-''hsp70'']]
−
|1.5 kb hsp70 promoter for heat-shock induction||4163||BM
+
|1.5 kb hsp70 promoter for heat-shock induction||4163||BM||1.0||
 
|-
 
|-
 
!327||[[p5E-UAS]]
 
!327||[[p5E-UAS]]
−
|10x UAS element and basal promoter for Gal4 response||3127||DSC
+
|10x UAS element and basal promoter for Gal4 response||3127||DSC||1.0||
 
|-
 
|-
 
!228||[[p5E-MCS]]
 
!228||[[p5E-MCS]]
−
|multi-cloning site from pBluescript||2810||BM
+
|multi-cloning site from pBluescript||2810||BM||1.0||
 
|-
 
|-
 
!381||[[p5E-Fse-Asc]]
 
!381||[[p5E-Fse-Asc]]
−
|restriction sites for 8-cutters FseI and AscI||2663||EF
+
|restriction sites for 8-cutters FseI and AscI||2663||EF||1.0||
  
 
|-
 
|-
−
! colspan="5" style="background:#ffdead;" |middle entry clones, attL1-L2 (kan resistant)
+
! colspan="7" style="background:#ffdead;" |middle entry clones, attL1-L2 (kan resistant)
 
|-
 
|-
 
!383||[[pME-EGFP]]
 
!383||[[pME-EGFP]]
−
|EGFP||3327||KMK
+
|EGFP||3327||KMK||1.0||
 
|-
 
|-
 
!384||[[pME-EGFPCAAX]]
 
!384||[[pME-EGFPCAAX]]
−
|membrane-localized (prenylated) EGFP; fused to 21 aa of H-ras||3345||KMK
+
|membrane-localized (prenylated) EGFP; fused to 21 aa of H-ras||3345||KMK||1.0||
 
|-
 
|-
 
!385||[[pME-nlsEGFP]]
 
!385||[[pME-nlsEGFP]]
−
|nuclear-localized EGFP||3342||KMK
+
|nuclear-localized EGFP||3342||KMK||1.0||
 
|-
 
|-
 
!386||[[pME-mCherry]]
 
!386||[[pME-mCherry]]
−
|monomeric red fluorophore mCherry||3261||KMK
+
|monomeric red fluorophore mCherry||3261||KMK||1.0||
 
|-
 
|-
 
!232||[[pME-mCherryCAAX]]
 
!232||[[pME-mCherryCAAX]]
−
|prenylated mCherry||3321||KMK
+
|prenylated mCherry||3321||KMK||1.0||
 
|-
 
|-
 
!233||[[pME-nlsmCherry]]
 
!233||[[pME-nlsmCherry]]
−
|nuclear-localized mCherry||3288||KMK
+
|nuclear-localized mCherry||3288||KMK||1.0||
 
|-
 
|-
 
!234||[[pME-H2AmCherry]]
 
!234||[[pME-H2AmCherry]]
−
|mCherry fused to zebrafish histone H2A.F/Z||3651||KMK
+
|mCherry fused to zebrafish histone H2A.F/Z||3651||KMK||1.0||
 
|-
 
|-
 
!387||[[pME-Gal4VP16]]
 
!387||[[pME-Gal4VP16]]
−
|Gal4 DNA binding domain fused to VP16 transactivation domain||3204||EF
+
|Gal4 DNA binding domain fused to VP16 transactivation domain||3204||EF||1.0||
  
 
|-
 
|-
−
! colspan="5" style="background:#ffdead;" |3' entry clones, attR2-L3 (kan resistant)
+
! colspan="7" style="background:#ffdead;" |3' entry clones, attR2-L3 (kan resistant)
 
|-
 
|-
 
!302||[[p3E-polyA]]
 
!302||[[p3E-polyA]]
−
|SV40 late polyA signal||2838||KMK
+
|SV40 late polyA signal||2838||KMK||1.0||
 
|-
 
|-
 
!229||[[p3E-MTpA]]
 
!229||[[p3E-MTpA]]
−
|6x myc tag for C-terminal fusions, plus SV40 late polyA||3151||BM
+
|6x myc tag for C-terminal fusions, plus SV40 late polyA||3151||BM||1.0||
 
|-
 
|-
 
!366||[[p3E-EGFPpA]]
 
!366||[[p3E-EGFPpA]]
−
|EGFP for C-terminal fusions, plus SV40 late polyA||3634||MEH
+
|EGFP for C-terminal fusions, plus SV40 late polyA||3634||MEH||1.0||
 
|-
 
|-
 
!388||[[p3E-mCherrypA]]
 
!388||[[p3E-mCherrypA]]
−
|mCherry for C-terminal fusions, plus SV40 late polyA||3586||MEH
+
|mCherry for C-terminal fusions, plus SV40 late polyA||3586||MEH||1.0||
 
|-
 
|-
 
!389||[[p3E-IRES-EGFPpA]]
 
!389||[[p3E-IRES-EGFPpA]]
−
|IRES driving EGFP plus SV40 late polyA||4219||KMK
+
|IRES driving EGFP plus SV40 late polyA||4219||KMK||1.0||
 
|-
 
|-
 
!390||[[p3E-IRES-EGFPCAAXpA]]
 
!390||[[p3E-IRES-EGFPCAAXpA]]
−
|IRES driving prenylated EGFP plus SV40 late polyA||4250||KMK
+
|IRES driving prenylated EGFP plus SV40 late polyA||4250||KMK||1.0||
 
|-
 
|-
 
!391||[[p3E-IRES-nlsEGFPpA]]
 
!391||[[p3E-IRES-nlsEGFPpA]]
−
|IRES driving nuclear EGFP plus SV40 late polyA||4248||KMK
+
|IRES driving nuclear EGFP plus SV40 late polyA||4248||KMK||1.0||
  
 
|-
 
|-
−
! colspan="5" style="background:#ffdead;" |destination vectors, attR4-R3 (amp/chlor resistant; grow in ccdB-tolerant cells)
+
! colspan="7" style="background:#ffdead;" |destination vectors, attR4-R3 (amp/chlor resistant; grow in ccdB-tolerant cells)
 
|-
 
|-
 
!392||[[pDestTol2pA]]*
 
!392||[[pDestTol2pA]]*
−
|attR4-R3 gate with SV40 polyA flanked by Tol2 inverted repeats||7897||CG
+
|attR4-R3 gate with SV40 polyA flanked by Tol2 inverted repeats||7903||CG||0.9||KK
 
|-
 
|-
 
!393||[[pDestTol2CG]]*
 
!393||[[pDestTol2CG]]*
−
|pDestTol2pA with cmlc2:egfp transgenesis marker||9702||EF
+
|pDestTol2pA with cmlc2:egfp transgenesis marker||9708||EF||0.9||KK
 
|-
 
|-
 
!394||[[pDestTol2pA2]]
 
!394||[[pDestTol2pA2]]
−
|pDestTol2pA with ~2 kb extraneous sequence removed||5877||EF
+
|pDestTol2pA with ~2 kb extraneous sequence removed||5883||EF||1.0||KK
 
|-
 
|-
 
!395||[[pDestTol2CG2]]
 
!395||[[pDestTol2CG2]]
−
|pDestTol2CG2 with ~2 kb extraneous sequence removed||7790||EF
+
|pDestTol2CG2 with ~2 kb extraneous sequence removed||7796||EF||1.0||KK
  
 
|-
 
|-
−
! colspan="5" style="background:#ffdead;" |transposase clones
+
! colspan="7" style="background:#ffdead;" |transposase clones
 
|-
 
|-
 
!396||[[pCS2FA-transposase]]
 
!396||[[pCS2FA-transposase]]
−
|for in vitro transcription of capped Tol2 transposase mRNA||6034||KMK
+
|for in vitro transcription of capped Tol2 transposase mRNA||6034||KMK||1.0||KK
 
|}
 
|}
 
'''Notes'''
 
'''Notes'''
Line 115: Line 115:
 
** KMK, Kristen Kwan, Chien lab
 
** KMK, Kristen Kwan, Chien lab
 
** MEH, Melissa Hardy, Chien lab
 
** MEH, Melissa Hardy, Chien lab
 +
* '''ver''' shows the version of the Tol2kit in which this construct first appeared.
 
* pDestTol2pA and pDestTol2CG have been superseded by pDestTol2pA2 and pDestTol2CG2. They are not formally part of the v1.0 release, and are no longer being distributed.
 
* pDestTol2pA and pDestTol2CG have been superseded by pDestTol2pA2 and pDestTol2CG2. They are not formally part of the v1.0 release, and are no longer being distributed.
 +
* '''MTA?''' indicates whether an MTA is associated with each clone. "KK", Koichi Kawakami.

Revision as of 18:07, 7 June 2007

Here is a list of the constructs in the Tol2kit.

Better-formatted information for all sequences is coming soon.

Constructs in the Tol2kit v1 (Feb 2007)

# name insert size (bp) made by ver MTA?
5' entry clones, attL4-R1 (kan resistant)
299 p5E-bactin2 5.3 kb beta-actin promoter (ubiquitous) 7950 KMK 1.0
380 p5E-h2afx 1 kb histone2A-X promoter (quasi-ubiquitous) 3604 KMK 1.0
382 p5E-CMV/SP6 1 kb CMV/SP6 cassette from pCS2+ 3704 KMK 1.0
222 p5E-hsp70 1.5 kb hsp70 promoter for heat-shock induction 4163 BM 1.0
327 p5E-UAS 10x UAS element and basal promoter for Gal4 response 3127 DSC 1.0
228 p5E-MCS multi-cloning site from pBluescript 2810 BM 1.0
381 p5E-Fse-Asc restriction sites for 8-cutters FseI and AscI 2663 EF 1.0
middle entry clones, attL1-L2 (kan resistant)
383 pME-EGFP EGFP 3327 KMK 1.0
384 pME-EGFPCAAX membrane-localized (prenylated) EGFP; fused to 21 aa of H-ras 3345 KMK 1.0
385 pME-nlsEGFP nuclear-localized EGFP 3342 KMK 1.0
386 pME-mCherry monomeric red fluorophore mCherry 3261 KMK 1.0
232 pME-mCherryCAAX prenylated mCherry 3321 KMK 1.0
233 pME-nlsmCherry nuclear-localized mCherry 3288 KMK 1.0
234 pME-H2AmCherry mCherry fused to zebrafish histone H2A.F/Z 3651 KMK 1.0
387 pME-Gal4VP16 Gal4 DNA binding domain fused to VP16 transactivation domain 3204 EF 1.0
3' entry clones, attR2-L3 (kan resistant)
302 p3E-polyA SV40 late polyA signal 2838 KMK 1.0
229 p3E-MTpA 6x myc tag for C-terminal fusions, plus SV40 late polyA 3151 BM 1.0
366 p3E-EGFPpA EGFP for C-terminal fusions, plus SV40 late polyA 3634 MEH 1.0
388 p3E-mCherrypA mCherry for C-terminal fusions, plus SV40 late polyA 3586 MEH 1.0
389 p3E-IRES-EGFPpA IRES driving EGFP plus SV40 late polyA 4219 KMK 1.0
390 p3E-IRES-EGFPCAAXpA IRES driving prenylated EGFP plus SV40 late polyA 4250 KMK 1.0
391 p3E-IRES-nlsEGFPpA IRES driving nuclear EGFP plus SV40 late polyA 4248 KMK 1.0
destination vectors, attR4-R3 (amp/chlor resistant; grow in ccdB-tolerant cells)
392 pDestTol2pA* attR4-R3 gate with SV40 polyA flanked by Tol2 inverted repeats 7903 CG 0.9 KK
393 pDestTol2CG* pDestTol2pA with cmlc2:egfp transgenesis marker 9708 EF 0.9 KK
394 pDestTol2pA2 pDestTol2pA with ~2 kb extraneous sequence removed 5883 EF 1.0 KK
395 pDestTol2CG2 pDestTol2CG2 with ~2 kb extraneous sequence removed 7796 EF 1.0 KK
transposase clones
396 pCS2FA-transposase for in vitro transcription of capped Tol2 transposase mRNA 6034 KMK 1.0 KK

Notes

  • # is the Chien lab stock number
  • made by shows who made the construct:
    • CG: Clemens Grabher, Look lab
    • BM: Ben Mangum, Chien lab
    • DSC, Doug Campbell, Chien lab
    • EF, Esther Fujimoto, Chien lab
    • KMK, Kristen Kwan, Chien lab
    • MEH, Melissa Hardy, Chien lab
  • ver shows the version of the Tol2kit in which this construct first appeared.
  • pDestTol2pA and pDestTol2CG have been superseded by pDestTol2pA2 and pDestTol2CG2. They are not formally part of the v1.0 release, and are no longer being distributed.
  • MTA? indicates whether an MTA is associated with each clone. "KK", Koichi Kawakami.
Retrieved from "http://tol2kit.genetics.utah.edu/index.php?title=List_of_entry_and_destination_vectors&oldid=254"