From Tol2Kit
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- 21:35, 18 April 2007 (diff | hist) . . (+37) . . Main Page (→About this site)
- 05:07, 18 April 2007 (diff | hist) . . (+56) . . N File:PDestTol2CG.png (Sequencher screenshot showing components of pDestTol2CG.) (current)
- 05:06, 18 April 2007 (diff | hist) . . (+21,422) . . N PDestTol2CG sequence (New page: <pre> >pDestTol2CG CACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAA TACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGC CCTTATTCCCTTTTTT...)
- 05:05, 18 April 2007 (diff | hist) . . (+604) . . N PDestTol2CG (New page: === Construction details === pDestTol2CG was made by PCRing the cmlc2:egfp-pA cassette, adding BglII sites in the primers, then cloning into the unique BglII site of pDestTol2pA and select...)
- 05:01, 18 April 2007 (diff | hist) . . (+18,833) . . PDestTol2pA sequence
- 04:56, 18 April 2007 (diff | hist) . . (+56) . . N File:PDestTol2pA.png (Sequencher screenshot showing components of pDestTol2pA.) (current)
- 04:54, 18 April 2007 (diff | hist) . . (+395) . . N PDestTol2pA (New page: === Construction details === pDestTol2pA was built by Clemens Grabher; we are in the process of asking him for construction details. === Crudely annotated sequence === FASTA file with the...)
- 04:53, 18 April 2007 (diff | hist) . . (+148) . . List of entry and destination vectors
- 02:51, 30 March 2007 (diff | hist) . . (+2) . . m Main Page (→Clone distribution)
- 03:34, 24 March 2007 (diff | hist) . . (+9) . . m PCS2FA-transposase (→Construction details)
- 03:33, 24 March 2007 (diff | hist) . . (+63) . . N File:PCS2FA-transposase.png (Sequencher screenshot showing components of pCS2FA-transposase.) (current)
- 03:32, 24 March 2007 (diff | hist) . . (+11,476) . . N PCS2FA-transposase sequence (New page: <pre> >pCS2FA-transposase TCGACCATAGCCAATTCAATATGGCGTATATGGACTCATGCCAATTCAAT ATGGTGGATCTGGACCTGTGCCAATTCAATATGGCGTATATGGACTCGTG CCAATTCAATATGGTGGATCTGGACCCCAGCCAATTCAATATGGCGGACT TGGCACCAT...) (current)
- 03:31, 24 March 2007 (diff | hist) . . (+748) . . N PCS2FA-transposase (New page: === Construction details === pCS2FA-transposase was made by PCRing the Tol2 transpoase cds from the original Kawakami clone, adding restriction sites, then conventionally subcloned into pC...)
- 03:26, 24 March 2007 (diff | hist) . . (-10) . . List of entry and destination vectors
- 03:24, 24 March 2007 (diff | hist) . . (+99) . . List of entry and destination vectors
- 03:12, 24 March 2007 (diff | hist) . . (+58) . . N File:P3E-mCherrypA.png (Sequencher screenshot showing components of p3E-mCherrypA.) (current)
- 03:11, 24 March 2007 (diff | hist) . . (+7,642) . . N P3E-mCherrypA sequence (New page: <pre> >p3E-mCherrypA CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTC...) (current)
- 03:11, 24 March 2007 (diff | hist) . . (+672) . . N P3E-mCherrypA (New page: === Construction details === p3E-mCherrypA was made by PCR amplification of mCherry and an SV40 late polyadenylation signal from a pCS2+-based construct, using primers to add att sites, fo...)
- 03:07, 24 March 2007 (diff | hist) . . (+55) . . N File:P3E-EGFPpA.png (Sequencher screenshot showing components of p3E-EGFPpA.) (current)
- 03:07, 24 March 2007 (diff | hist) . . (+32) . . P3E-EGFPpA (→Construction details)
- 03:06, 24 March 2007 (diff | hist) . . (+7,836) . . N P3E-EGFPpA sequence (New page: <pre> >p3E_EGFPpA CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATT...) (current)
- 03:05, 24 March 2007 (diff | hist) . . (+714) . . N P3E-EGFPpA (New page: === Construction details === p3E-EGFPpA was made by PCR amplification of EGFP and an SV40 late polyadenylation signal from a pCS2+-based plasmid, using primers to add att sites, followed b...)
- 03:03, 24 March 2007 (diff | hist) . . (+5) . . m P3E-MTpA (→Construction details)
- 03:02, 24 March 2007 (diff | hist) . . (+53) . . N File:P3E-MTpA.png (Sequencher screenshot showing components of p3E-MTpA.) (current)
- 03:02, 24 March 2007 (diff | hist) . . (0) . . m P3E-MTpA (→Construction details)
- 03:01, 24 March 2007 (diff | hist) . . (+6,300) . . N P3E-MTpA sequence (New page: <pre> >p3E_MT-pA CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTA...) (current)
- 03:00, 24 March 2007 (diff | hist) . . (+507) . . N P3E-MTpA (New page: === Construction details === p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 polyadenylation signal from pCS2-MT, using primers to add att sequences, followed by a BP reaction....)
- 02:35, 24 March 2007 (diff | hist) . . (0) . . m P3E-polyA (→Construction details)
- 02:34, 24 March 2007 (diff | hist) . . (+54) . . N File:P3E-polyA.png (Sequencher screenshot showing components of p3E-polyA.) (current)
- 02:32, 24 March 2007 (diff | hist) . . (+6) . . P3E-polyA sequence (current)
- 02:32, 24 March 2007 (diff | hist) . . (+5,363) . . N P3E-polyA sequence (New page: <pre> >p3E-polyA CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTA...)
- 02:31, 24 March 2007 (diff | hist) . . (+501) . . N P3E-polyA (New page: === Construction details === p3E-polyA was made by PCR amplificaiton of the SV40 late polyadenylation signal from pCS2+, using primers that add att sites, followed by a BP reaction. Sequen...)
- 02:18, 24 March 2007 (diff | hist) . . (+57) . . N File:PME-Gal4VP16.png (Sequencher screenshot showing components of pME-Gal4VP16.) (current)
- 02:18, 24 March 2007 (diff | hist) . . (+63) . . m PME-Gal4VP16 (→Construction details)
- 02:16, 24 March 2007 (diff | hist) . . (+6,586) . . N PME-Gal4VP16 sequence (New page: <pre> >pME_Gal4VP16 CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCA...) (current)
- 02:16, 24 March 2007 (diff | hist) . . (+460) . . N PME-Gal4VP16 (New page: === Construction details === pME-Gal4VP16 was made by PCR amplification of Gal4VP16, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation,...)
- 02:14, 24 March 2007 (diff | hist) . . (+59) . . N File:PME-H2AmCherry.png (Sequencher screenshot showing components of pME-H2AmCherry.) (current)
- 02:13, 24 March 2007 (diff | hist) . . (+7,973) . . N PME-H2AmCherry sequence (New page: <pre> >pME-H2AF/Z-mCherry CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCC...) (current)
- 02:13, 24 March 2007 (diff | hist) . . (+520) . . N PME-H2AmCherry (New page: === Construction details === pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR) to the N terminus of mCherry, using primers that add att sites, followed by a...)
- 02:06, 24 March 2007 (diff | hist) . . (+59) . . N File:PME-nlsmCherry.png (Sequencher screenshot showing components of pME-nlsmCherry.) (current)
- 02:06, 24 March 2007 (diff | hist) . . (+6,864) . . N PME-nlsmCherry sequence (New page: <pre> >pME-nlsmCherry CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATT...) (current)
- 02:04, 24 March 2007 (diff | hist) . . (+142) . . PME-nlsmCherry
- 02:02, 24 March 2007 (diff | hist) . . (+536) . . N PME-nlsmCherry (New page: === Construction details === pME-nlsmCherry was made by PCR amplification of an nls-mCherry (mCherry with nuclear-localization signal) cassette, using primers that add att sites, followed ...)
- 01:59, 24 March 2007 (diff | hist) . . (+60) . . N File:PME-mCherryCAAX.png (Sequencher screenshot showing components of pME-mCherryCAAX.) (current)
- 01:58, 24 March 2007 (diff | hist) . . (+7,001) . . N PME-mCherryCAAX H80D sequence (New page: <pre> >pME-mCherryCAAX CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGAT...)
- 01:57, 24 March 2007 (diff | hist) . . (+601) . . N PME-mCherryCAAX H80D (New page: === Construction details === pME-mCherryCAAX was made by PCR amplification of mCherry-CAAX, using primers to add att sites, followed by a BP reaction. mCherry-CAAX is mCherry with a C-term...)
- 01:54, 24 March 2007 (diff | hist) . . (+56) . . N File:PME-mCherry.png (Sequencher screenshot showing components of pME-mCherry.) (current)
- 01:53, 24 March 2007 (diff | hist) . . (+6,771) . . N PME-mCherry sequence (New page: <pre> >pME-mCherry CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCAT...) (current)
- 01:52, 24 March 2007 (diff | hist) . . (+468) . . N PME-mCherry (New page: === Construction details === pME-mCherry was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the i...)
- 01:51, 24 March 2007 (diff | hist) . . (+16) . . m PME-nlsEGFP (→Construction details)
- 01:50, 24 March 2007 (diff | hist) . . (+56) . . N File:PME-nlsEGFP.png (Sequencher screenshot showing components of pME-nlsEGFP.) (current)
- 01:49, 24 March 2007 (diff | hist) . . (+6,970) . . N PME-nlsEGFP sequence (New page: <pre> >pME-nlsEGFP CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCAT...) (current)
- 01:48, 24 March 2007 (diff | hist) . . (+505) . . N PME-nlsEGFP (New page: === Construction details === pME-nlsEGFP was made by PCR amplification of an nls-EGFP (nuclear-localized GFP) cassette, using primers that add att sites, followed by a BP reaction. Sequen...)
- 01:46, 24 March 2007 (diff | hist) . . (+57) . . N File:PME-EGFPCAAX.png (Sequencher screenshot showing components of pME-EGFPCAAX.) (current)
- 01:45, 24 March 2007 (diff | hist) . . (+7,079) . . N PME-EGFPCAAX sequence (New page: <pre> >pME-EGFP-CAAX CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTC...) (current)
- 01:44, 24 March 2007 (diff | hist) . . (+583) . . N PME-EGFPCAAX (New page: === Construction details === pME-EGFPCAAX was made by PCR amplification of EGFP-CAAX, using primers to add att sites, followed by a BP reaction. EGFP-CAAX is EGFP with a C-terminal fusion ...)
- 01:40, 24 March 2007 (diff | hist) . . (+37) . . m PME-EGFP (→Construction details)
- 01:39, 24 March 2007 (diff | hist) . . (+1) . . PME-EGFP (→Construction details)
- 01:38, 24 March 2007 (diff | hist) . . (+186) . . PME-EGFP (→Construction details)
- 01:35, 24 March 2007 (diff | hist) . . (+53) . . N File:PME-EGFP.png (Sequencher screenshot showing components of pME-EGFP.) (current)
- 01:34, 24 March 2007 (diff | hist) . . (+7,002) . . N PME-EGFP sequence (New page: <pre> >pME-EGFP CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTAA...) (current)
- 01:32, 24 March 2007 (diff | hist) . . (+456) . . N PME-EGFP (New page: === Construction details === pME-EGFP was made by PCR amplification of EGFP, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert ...)
- 01:27, 24 March 2007 (diff | hist) . . (+52) . . List of entry and destination vectors
- 03:53, 23 March 2007 (diff | hist) . . (+41) . . P5E-UAS (→Construction details)
- 03:52, 23 March 2007 (diff | hist) . . (+52) . . N File:P5E-UAS.png (Sequencher screenshot showing components of p5E-UAS.) (current)
- 03:51, 23 March 2007 (diff | hist) . . (+6,169) . . N P5E-UAS sequence (New page: <pre> >p5E_UAS CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTAAT...)
- 03:51, 23 March 2007 (diff | hist) . . (+997) . . N P5E-UAS (New page: === Construction details === p5E-UAS was made by conventionally subcloning into p5E-MCS a HindIII-EcoRI fragment from a UAS-E1b construct from Reinhard Köster, containing multimerized Gal...)
- 03:33, 23 March 2007 (diff | hist) . . (+19) . . m P5E-hsp70l (→Construction details)
- 03:32, 23 March 2007 (diff | hist) . . (+58) . . N File:P5E-hsp70.png (Sequencher screenshot showing components of p5E-''hsp70''.) (current)
- 03:31, 23 March 2007 (diff | hist) . . (+9,424) . . N P5E-hsp70l sequence (New page: <pre> >p5E-hsp70 CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTA...)
- 03:31, 23 March 2007 (diff | hist) . . (+597) . . N P5E-hsp70l (New page: === Construction details === p5E-''hsp70'' was made by PCR amplification of the 1.5 kb hsp70 promoter, using primers to add att sites, followed by a BP reaction. Sequencing shows a single...)
- 03:24, 23 March 2007 (diff | hist) . . (+56) . . N File:P5E-CMV-SP6.png (Sequencher screenshot showing components of p5E-CMV/SP6.) (current)
- 03:23, 23 March 2007 (diff | hist) . . (+7,957) . . N P5E-CMV-SP6 sequence (New page: <pre> >p5E-CMV-SP6 CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCAT...) (current)
- 03:22, 23 March 2007 (diff | hist) . . (+821) . . N P5E-CMV/SP6 (New page: === Construction details === p5E-CMV/SP6 was made by PCR amplification of the sCMV and SP6 promoters from pCS2+, using primers that added att sites, followed by a BP reaction. This 5' el...)
- 22:29, 22 March 2007 (diff | hist) . . (+58) . . N File:P5E-h2afx.png (Sequencher screenshot showing components of p5E-''h2afx''.) (current)
- 22:28, 22 March 2007 (diff | hist) . . (+7,753) . . N P5E-h2afx sequence (New page: <pre> >p5E-h2afx CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTA...) (current)
- 22:27, 22 March 2007 (diff | hist) . . (+1,008) . . N P5E-h2afx (New page: === Construction details === p5E-''h2afx'' was made by PCR amplification of 1 kb genomic DNA immediately upstream of the start codon of ''h2afx'' (NM_201073), using primers that added att ...)
- 22:09, 22 March 2007 (diff | hist) . . (+36) . . List of entry and destination vectors
- 21:35, 22 March 2007 (diff | hist) . . (+822) . . Main Page
- 23:20, 19 March 2007 (diff | hist) . . (+371) . . Protocols
- 23:16, 19 March 2007 (diff | hist) . . (+82) . . List of entry and destination vectors
- 23:14, 19 March 2007 (diff | hist) . . (+152) . . P5E-MCS sequence (current)
- 23:13, 19 March 2007 (diff | hist) . . (-2,735) . . P5E-Fse-Asc sequence (current)
- 23:12, 19 March 2007 (diff | hist) . . (-2,731) . . P3E-IRES-EGFPpA sequence (current)
- 23:11, 19 March 2007 (diff | hist) . . (-3,565) . . P3E-IRES-EGFPCAAXpA sequence (current)
- 23:10, 19 March 2007 (diff | hist) . . (-2,738) . . P3E-IRES-nlsEGFPpA sequence (current)
- 23:08, 19 March 2007 (diff | hist) . . (-1,362) . . PDestTol2pA2 sequence
- 23:05, 19 March 2007 (diff | hist) . . (-1,393) . . PDestTol2CG2 sequence
- 23:00, 19 March 2007 (diff | hist) . . (-6) . . m PDestTol2CG2
- 22:59, 19 March 2007 (diff | hist) . . (-6) . . m PDestTol2pA2
- 22:58, 19 March 2007 (diff | hist) . . (-6) . . m P3E-IRES-nlsEGFPpA
- 22:57, 19 March 2007 (diff | hist) . . (-6) . . m P3E-IRES-EGFPCAAXpA
- 22:57, 19 March 2007 (diff | hist) . . (-6) . . m P3E-IRES-EGFPpA
- 22:56, 19 March 2007 (diff | hist) . . (-6) . . m P5E-Fse-Asc
- 22:55, 19 March 2007 (diff | hist) . . (-6) . . P5E-MCS
- 22:53, 19 March 2007 (diff | hist) . . (+56) . . N File:P5E-bactin2.png (Sequencher screenshot showing components of p5E-bactin2.) (current)
- 22:52, 19 March 2007 (diff | hist) . . (+22,557) . . N P5E-bactin2 sequence (New page: <pre> >p5E-bactin2 CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCAT...) (current)
- 21:00, 19 March 2007 (diff | hist) . . (+1,098) . . N P5E-bactin2 (New page: === Construction details === ''bactin2'' genomic sequence was derived from a 5.3 kb clone (gift of Ken Poss), which was derived in turn from a 10 kb upstream construct from Shin-ichi Higas...)
- 20:51, 19 March 2007 (diff | hist) . . (+16) . . List of entry and destination vectors
- 14:34, 24 February 2007 (diff | hist) . . (+3,536) . . N Att site sequences (New page: == att site shared sequences == Here is a set of "shared" sequences--the core stretch that is shared between attB/P/L/R for att1-4. These are useful for stitching together entry and destin...)
- 14:21, 24 February 2007 (diff | hist) . . (+39) . . m Protocols (→What you will need)
- 14:58, 23 February 2007 (diff | hist) . . (-12) . . m Protocols (→BP Reactions)
- 13:20, 23 February 2007 (diff | hist) . . (+300) . . Protocols
- 13:12, 23 February 2007 (diff | hist) . . (+259) . . Protocols
- 17:10, 21 February 2007 (diff | hist) . . (-56) . . m Protocols (→What you will need)
- 20:42, 20 February 2007 (diff | hist) . . (+229) . . List of entry and destination vectors
- 19:29, 20 February 2007 (diff | hist) . . (+192) . . Protocols (→What you will need)
- 19:27, 20 February 2007 (diff | hist) . . (+70) . . Protocols (→LR reactions)
- 19:19, 20 February 2007 (diff | hist) . . (+3) . . Protocols (→BP Reactions)
- 19:18, 20 February 2007 (diff | hist) . . (+399) . . Protocols (→BP Reactions)
- 19:10, 20 February 2007 (diff | hist) . . (+245) . . Protocols (→What you will need)
- 12:39, 16 February 2007 (diff | hist) . . (+161) . . Protocols
- 00:28, 13 February 2007 (diff | hist) . . (+97) . . Protocols
- 00:20, 13 February 2007 (diff | hist) . . (-2) . . m Protocols (→Growing up clones)
- 00:18, 13 February 2007 (diff | hist) . . (+990) . . Protocols
- 00:03, 13 February 2007 (diff | hist) . . (+56) . . N File:P5E-Fse-Asc.png (Sequencher screenshot showing components of p5E-Fse-Asc.) (current)
- 00:02, 13 February 2007 (diff | hist) . . (+7,528) . . N P5E-Fse-Asc sequence (New page: <pre> >p5E-Fse-Asc CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCAT...)
- 00:01, 13 February 2007 (diff | hist) . . (+616) . . N P5E-Fse-Asc (New page: '''Construction details'''<br> p5E-Fse-Asc was made by PCR of two overlapping primers with att sites and the Fse and Asc recognition sites, followed by a BP reaction. The insert consists o...)
- 23:56, 12 February 2007 (diff | hist) . . (+52) . . N File:P5E-MCS.png (Sequencher screenshot showing components of p5E-MCS.) (current)
- 23:55, 12 February 2007 (diff | hist) . . (+464) . . N P5E-MCS (New page: '''Construction details'''<br> p5E-MCS was made by PCR of the pBSII SK+ multiple cloning site, from T7 to T3 primers, followed by a BP reaction. Sequencing confirms that all bases of the i...)
- 23:51, 12 February 2007 (diff | hist) . . (+5,187) . . N P5E-MCS sequence (New page: <pre> >p5E_MCS CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTAAT...)
- 23:46, 12 February 2007 (diff | hist) . . (+8) . . List of entry and destination vectors
- 19:44, 7 February 2007 (diff | hist) . . (+63) . . N File:P3E-IRES-nlsEGFPpA.png (Sequencher screenshot showing components of p3E-IRES-nlsEGFPpA.) (current)
- 19:43, 7 February 2007 (diff | hist) . . (-1) . . m P3E-IRES-nlsEGFPpA
- 19:43, 7 February 2007 (diff | hist) . . (+12,404) . . N P3E-IRES-nlsEGFPpA sequence (New page: <pre> >p3E-IRES-nlsEGFPpA CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCC...)
- 19:42, 7 February 2007 (diff | hist) . . (+662) . . N P3E-IRES-nlsEGFPpA (New page: '''Construction details'''<br> p3E-IRES-nlsEGFPpA was made by fusion PCR of the EMCV IRES sequence, and nlsEGFP-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all bas...)
- 19:40, 7 February 2007 (diff | hist) . . (+1) . . m P3E-IRES-EGFPCAAXpA
- 19:39, 7 February 2007 (diff | hist) . . (+64) . . N File:P3E-IRES-EGFPCAAXpA.png (Sequencher screenshot showing components of p3E-IRES-EGFPCAAXpA.) (current)
- 19:39, 7 February 2007 (diff | hist) . . (+13,299) . . N P3E-IRES-EGFPCAAXpA sequence (New page: <pre> >p3E-IRES-EGFPCAAXpA CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGC...)
- 19:38, 7 February 2007 (diff | hist) . . (+500) . . N P3E-IRES-EGFPCAAXpA (New page: ''Construction details'''<br> p3E-IRES-EGFPCAAXpA was made by fusion PCR of the EMCV IRES sequence, and EGFPCAAX-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all ba...)
- 19:31, 7 February 2007 (diff | hist) . . (+186) . . P3E-IRES-EGFPpA
- 19:30, 7 February 2007 (diff | hist) . . (+94) . . P3E-IRES-EGFPpA sequence
- 19:25, 7 February 2007 (diff | hist) . . (+60) . . N File:P3E-IRES-EGFPpA.png (Sequencher screenshot showing components of p3E-IRES-EGFPpA.) (current)
- 19:24, 7 February 2007 (diff | hist) . . (+12,308) . . N P3E-IRES-EGFPpA sequence (New page: <pre> >p3E-IRES-EGFPpA CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGAT...)
- 19:21, 7 February 2007 (diff | hist) . . (+486) . . N P3E-IRES-EGFPpA (New page: '''Construction details'''<br> p3E-IRES-EGFPpA was made by fusion PCR of the EMCV IRES sequence, and EGFP-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all bases of ...)
- 19:17, 7 February 2007 (diff | hist) . . (+1) . . File:PDestTol2pA2.png (current)
- 19:16, 7 February 2007 (diff | hist) . . (+57) . . N File:PDestTol2CG2.png (Sequencher screenshot showing components of pDestTol2CG2.) (current)
- 19:15, 7 February 2007 (diff | hist) . . (+19,035) . . N PDestTol2CG2 sequence (New page: <pre> >pDestTol2CG2 CACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAA TACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGC CCTTATTCCCTTTTT...)
- 19:14, 7 February 2007 (diff | hist) . . (+546) . . N PDestTol2CG2 (New page: '''Construction details'''<br> pDestTol2CG2 was made from pDestTol2CG by cutting with AfeII/NgoMI and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part o...)
- 19:12, 7 February 2007 (diff | hist) . . (0) . . m PDestTol2pA2 sequence (PDestTol2pA sequence moved to PDestTol2pA2 sequence: typo in original name)
- 19:12, 7 February 2007 (diff | hist) . . (+35) . . N PDestTol2pA sequence (PDestTol2pA sequence moved to PDestTol2pA2 sequence: typo in original name)
- 19:11, 7 February 2007 (diff | hist) . . (+28) . . PDestTol2pA2
- 19:09, 7 February 2007 (diff | hist) . . (-1) . . m List of entry and destination vectors
- 19:08, 7 February 2007 (diff | hist) . . (+84) . . List of entry and destination vectors
- 19:06, 7 February 2007 (diff | hist) . . (+56) . . N File:PDestTol2pA2.png (Sequencher screenshot showing components of pDestTol2pA.)
- 19:05, 7 February 2007 (diff | hist) . . (+16,113) . . N PDestTol2pA2 sequence (New page: <pre> >pDest-Tol2-pA_v2 CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT GGAACAAGAGT...)
- 19:00, 7 February 2007 (diff | hist) . . (+519) . . N PDestTol2pA2 (New page: '''Construction details'''<br> pDestTol2pA2 was made from pDestTol2pA by cutting with AfeII/DraIII and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part ...)
- 19:08, 6 February 2007 (diff | hist) . . (+66) . . List of entry and destination vectors
- 13:11, 2 February 2007 (diff | hist) . . (+217) . . Main Page
- 13:05, 2 February 2007 (diff | hist) . . (+104) . . Main Page
- 13:03, 2 February 2007 (diff | hist) . . (+187) . . List of entry and destination vectors
- 12:46, 2 February 2007 (diff | hist) . . (0) . . Basic Gateway principles (→Principles of Gateway recombination)
- 12:40, 2 February 2007 (diff | hist) . . (+37) . . Basic Gateway principles (→Principles of Gateway recombination)
- 12:40, 2 February 2007 (diff | hist) . . (+166) . . N File:BP reaction.png (Diagram of a Gateway BP reaction. A "middle" clone (pME) is constructed using PCR with gene-specific primers to add att sites, then recombination with a donor vector.)
- 12:39, 2 February 2007 (diff | hist) . . (+208) . . Basic Gateway principles (→Principles of Gateway recombination)
- 12:36, 2 February 2007 (diff | hist) . . (+142) . . Main Page
- 05:20, 2 February 2007 (diff | hist) . . (+52) . . Protocols (→LR reactions)
- 05:19, 2 February 2007 (diff | hist) . . (-21) . . File:Clear-opaque.png (current)
- 05:18, 2 February 2007 (diff | hist) . . (+129) . . N File:Clear-opaque.png (Image of colonies from LR reaction showing three clear (correct) colonies (arrowheads) and one opaque (incorrect) colony (arrow).)
- 19:52, 31 January 2007 (diff | hist) . . (-4) . . Basic Gateway principles
- 19:45, 31 January 2007 (diff | hist) . . (-1) . . Basic Gateway principles
- 19:35, 31 January 2007 (diff | hist) . . (-5) . . Basic Gateway principles
- 19:35, 31 January 2007 (diff | hist) . . (0) . . Basic Gateway principles
- 19:30, 31 January 2007 (diff | hist) . . (+6) . . Basic Gateway principles
- 19:16, 31 January 2007 (diff | hist) . . (-6) . . Basic Gateway principles
- 19:15, 31 January 2007 (diff | hist) . . (+18) . . Basic Gateway principles
- 19:06, 31 January 2007 (diff | hist) . . (+168) . . Basic Gateway principles
- 19:00, 31 January 2007 (diff | hist) . . (+108) . . N File:LR reaction.png (Diagram of a multisite Gateway LR reaction, combining 3 entry clones with the destination vector pDestTol2pA)
- 13:55, 31 January 2007 (diff | hist) . . (+218) . . List of entry and destination vectors
- 13:53, 31 January 2007 (diff | hist) . . (+6) . . List of entry and destination vectors
- 13:38, 31 January 2007 (diff | hist) . . (+66) . . List of entry and destination vectors
- 13:33, 31 January 2007 (diff | hist) . . (+2,506) . . N List of entry and destination vectors (New page: This is a test of table features... {| |+ '''Constructs in the Tol2kit v1 (Jan 2007)''' ! name !! # !! insert !! made by |- ! colspan="4" style="background:#ffdead;" |5' entry clones, ...)
- 17:35, 30 January 2007 (diff | hist) . . (+25) . . N References (New page: Coming some day. 1/30/07.) (current)
- 17:35, 30 January 2007 (diff | hist) . . (+30) . . N Sample results with the Tol2kit (New page: Coming at some point, 1/30/07.)
- 17:34, 30 January 2007 (diff | hist) . . (-15) . . Sources
- 17:33, 30 January 2007 (diff | hist) . . (+279) . . N Sources (New page: Coming soon 1/30/07. This page will talk about the provenance of some components that we have used in the Tol2kit: * fluorescent proteins (FPs): EGFP, mCherry * fusion tags used for FP su...)
- 17:30, 30 January 2007 (diff | hist) . . (+55) . . Main Page (→About this site)
- 17:29, 30 January 2007 (diff | hist) . . (+114) . . N Basic Gateway principles (New page: (content is coming soon. 1/30/07) == Principles of Gateway recombination == == How we use this in the Tol2kit ==)
- 17:27, 30 January 2007 (diff | hist) . . (+295) . . Main Page
- 17:23, 30 January 2007 (diff | hist) . . (-30) . . Main Page (→'''Welcome to the Tol2kit documentation site''')
- 17:22, 30 January 2007 (diff | hist) . . (-64) . . Main Page (→'''Welcome to the Tol2kit documentation site''')
- 17:21, 30 January 2007 (diff | hist) . . (+1,067) . . Main Page (→'''Welcome to the Tol2kit documentation site''')
- 04:35, 30 January 2007 (diff | hist) . . (+6) . . Main Page
- 04:35, 30 January 2007 (diff | hist) . . (+289) . . Main Page
- 03:06, 30 January 2007 (diff | hist) . . (-68) . . Main Page
- 00:35, 30 January 2007 (diff | hist) . . (+594) . . N Tol2Kit:About (New page: A multisite Gateway-based construction kit for building zebrafish expression vectors in a Tol2 transposon backbone. The constructs in the initial release of the Tol2kit (Jan 2007) were bu...) (current)
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