From Tol2Kit
- 18:49, 17 August 2011 (diff | hist) . . (+22) . . P3E-IRES-EGFPCAAXpA (→Construction details) (current)
- 18:48, 17 August 2011 (diff | hist) . . (+22) . . P3E-IRES-EGFPpA (→Construction details) (current)
- 18:47, 17 August 2011 (diff | hist) . . (+22) . . P3E-IRES-nlsEGFPpA (→Construction details) (current)
- 03:14, 18 December 2010 (diff | hist) . . (+35) . . Protocols (reformatting)
- 03:10, 18 December 2010 (diff | hist) . . (+689) . . Protocols (added basepair changes in attL1 and attP1)
- 17:24, 7 March 2010 (diff | hist) . . (+303) . . PME-Gal4VP16 (→Construction details) (current)
- 17:18, 7 March 2010 (diff | hist) . . (+301) . . Main Page (→Revision history)
- 17:15, 7 March 2010 (diff | hist) . . (+37) . . PME-Gal4VP16 (→Construction details)
- 19:19, 22 January 2010 (diff | hist) . . (+179) . . PME-MCS (→Sequence) (current)
- 19:16, 22 January 2010 (diff | hist) . . (+161) . . PME-MCS (→Sequence)
- 11:59, 12 October 2009 (diff | hist) . . (+191) . . Main Page (→Revision history)
- 11:56, 12 October 2009 (diff | hist) . . (0) . . PME-mCherryCAAX Genbank (current)
- 11:55, 12 October 2009 (diff | hist) . . (+10) . . PME-mCherryCAAX (current)
- 11:52, 12 October 2009 (diff | hist) . . (+142) . . PME-mCherryCAAX (→Construction details)
- 11:50, 12 October 2009 (diff | hist) . . (-2) . . List of entry and destination vectors (current)
- 11:50, 12 October 2009 (diff | hist) . . (+136) . . List of entry and destination vectors
- 11:48, 12 October 2009 (diff | hist) . . (+122) . . List of entry and destination vectors
- 04:07, 7 May 2009 (diff | hist) . . (+4) . . Main Page (→Revision history)
- 04:06, 7 May 2009 (diff | hist) . . (+137) . . Main Page (→Revision history)
- 04:04, 7 May 2009 (diff | hist) . . (-6) . . Main Page (→Citing the Tol2kit)
- 14:35, 28 April 2009 (diff | hist) . . (0) . . List of entry and destination vectors
- 03:04, 25 April 2009 (diff | hist) . . (-8) . . List of entry and destination vectors
- 17:44, 23 August 2008 (diff | hist) . . (+188) . . PME-mCherryCAAX (→Construction details)
- 17:34, 22 August 2008 (diff | hist) . . (0) . . P5E-bactin2 (→Construction details) (current)
- 16:08, 11 April 2008 (diff | hist) . . (-1) . . P5E-UAS sequence (current)
- 12:12, 1 April 2008 (diff | hist) . . (+1) . . List of entry and destination vectors
- 12:03, 1 April 2008 (diff | hist) . . (+14) . . P5E-hsp70l (→Construction details) (current)
- 12:03, 1 April 2008 (diff | hist) . . (-1) . . P5E-hsp70l (→Construction details)
- 12:02, 1 April 2008 (diff | hist) . . (-10) . . P5E-hsp70l (→Construction details)
- 12:02, 1 April 2008 (diff | hist) . . (+3) . . P5E-hsp70l sequence (current)
- 12:01, 1 April 2008 (diff | hist) . . (+2) . . P5E-hsp70l Genbank (current)
- 12:00, 1 April 2008 (diff | hist) . . (+189) . . P5E-hsp70l
- 11:59, 1 April 2008 (diff | hist) . . (0) . . m P5E-hsp70l sequence (P5E-hsp70 sequence moved to P5E-hsp70l sequence: Changing name to reflect official ZFIN gene name "hsp70l".)
- 11:59, 1 April 2008 (diff | hist) . . (+33) . . N P5E-hsp70 sequence (P5E-hsp70 sequence moved to P5E-hsp70l sequence: Changing name to reflect official ZFIN gene name "hsp70l".) (current)
- 11:58, 1 April 2008 (diff | hist) . . (+32) . . N P5E-hsp70 Genbank (P5E-hsp70 Genbank moved to P5E-hsp70l Genbank: Changing name to reflect official ZFIN gene name "hsp70l".) (current)
- 11:58, 1 April 2008 (diff | hist) . . (0) . . m P5E-hsp70l Genbank (P5E-hsp70 Genbank moved to P5E-hsp70l Genbank: Changing name to reflect official ZFIN gene name "hsp70l".)
- 11:57, 1 April 2008 (diff | hist) . . (0) . . m P5E-hsp70l (P5E-hsp70 moved to P5E-hsp70l: Changing name to match official ZFIN gene name)
- 11:57, 1 April 2008 (diff | hist) . . (+24) . . N P5E-hsp70 (P5E-hsp70 moved to P5E-hsp70l: Changing name to match official ZFIN gene name) (current)
- 11:56, 1 April 2008 (diff | hist) . . (-1) . . List of entry and destination vectors
- 11:55, 1 April 2008 (diff | hist) . . (+3) . . List of entry and destination vectors
- 11:55, 1 April 2008 (diff | hist) . . (+35) . . PME-H2AmCherry (→Construction details) (current)
- 04:42, 19 December 2007 (diff | hist) . . (+143) . . Main Page (→Revision history)
- 04:40, 19 December 2007 (diff | hist) . . (-94) . . MediaWiki:Sidebar (current)
- 04:37, 19 December 2007 (diff | hist) . . (0) . . MediaWiki:Sidebar
- 04:34, 19 December 2007 (diff | hist) . . (+38) . . MediaWiki:Sidebar
- 04:33, 19 December 2007 (diff | hist) . . (-36) . . MediaWiki:Sidebar
- 04:33, 19 December 2007 (diff | hist) . . (+56) . . N Sandbox (New page: Protocols#Assembling sequences for expression clones) (current)
- 04:32, 19 December 2007 (diff | hist) . . (+36) . . MediaWiki:Sidebar
- 04:29, 19 December 2007 (diff | hist) . . (+829) . . Protocols (→Assembling sequences for expression clones)
- 04:19, 19 December 2007 (diff | hist) . . (+87) . . Main Page (→Revision history)
- 04:18, 19 December 2007 (diff | hist) . . (-1) . . m List of entry and destination vectors
- 04:18, 19 December 2007 (diff | hist) . . (+73) . . List of entry and destination vectors
- 04:18, 19 December 2007 (diff | hist) . . (+9,907) . . PCS2FA-transposase Genbank (current)
- 04:15, 19 December 2007 (diff | hist) . . (+12) . . N PCS2FA-transposase Genbank (New page: <pre> </pre>)
- 04:14, 19 December 2007 (diff | hist) . . (+54) . . PCS2FA-transposase (current)
- 04:12, 19 December 2007 (diff | hist) . . (+14,583) . . N PDestTol2CG2 Genbank (New page: <pre> LOCUS 395.pDestTol2CG2 7796 bp ds-DNA circular 10-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant...) (current)
- 04:12, 19 December 2007 (diff | hist) . . (+11,547) . . N PDestTol2pA2 Genbank (New page: <pre> LOCUS 394.pDestTol2pA2 5883 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant...) (current)
- 04:11, 19 December 2007 (diff | hist) . . (+8,737) . . N P3E-IRES-nlsEGFPpA Genbank (New page: <pre> LOCUS 391.p3E-IRES-nlsEGFPpA 4248 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, ...) (current)
- 04:11, 19 December 2007 (diff | hist) . . (+8,763) . . N P3E-IRES-EGFPCAAXpA Genbank (New page: <pre> LOCUS 390.p3E-IRES-EGFPCAAXpA 4250 bp ds-DNA circular 10-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, ...) (current)
- 04:10, 19 December 2007 (diff | hist) . . (+8,717) . . N P3E-IRES-EGFPpA Genbank (New page: <pre> LOCUS 389.p3E-IRES-EGFPpA 4219 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Par...) (current)
- 04:10, 19 December 2007 (diff | hist) . . (+7,243) . . N P3E-mCherrypA Genbank (New page: <pre> LOCUS 388.p3E-mCherrypA 3586 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Paran...) (current)
- 04:09, 19 December 2007 (diff | hist) . . (+7,621) . . N P3E-EGFPpA Genbank (New page: <pre> LOCUS 366.p3E-EGFPpA 3634 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant J...) (current)
- 04:09, 19 December 2007 (diff | hist) . . (+6,663) . . N P3E-MTpA Genbank (New page: <pre> LOCUS 229.p3E-MT-pA 3151 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant JM...) (current)
- 04:08, 19 December 2007 (diff | hist) . . (+6,106) . . N P3E-polyA Genbank (New page: <pre> LOCUS 302.p3E-polyA 2838 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant JM...) (current)
- 04:08, 19 December 2007 (diff | hist) . . (+6,651) . . N PME-mCherry no stop Genbank (New page: <pre> LOCUS 456.pME-mCherry-no stop 3258 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, ...) (current)
- 04:08, 19 December 2007 (diff | hist) . . (+6,888) . . N PME-EGFP no stop Genbank (New page: <pre> LOCUS 455.pME-EGFP-no stop 3324 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Pa...) (current)
- 04:07, 19 December 2007 (diff | hist) . . (+6,900) . . N PME-mCherryCAAX Genbank (New page: <pre> LOCUS 450.pME-mCherryCAAX 3321 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Para...)
- 04:06, 19 December 2007 (diff | hist) . . (+6,527) . . N PME-MCS Genbank (New page: <pre> LOCUS 237.pME-MCS 2765 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant JM, ...) (current)
- 04:06, 19 December 2007 (diff | hist) . . (+6,737) . . N PME-Gal4VP16 Genbank (New page: <pre> LOCUS 387.Gal4VP16 3204 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant JM,...) (current)
- 04:05, 19 December 2007 (diff | hist) . . (+7,297) . . N PME-H2AmCherry Genbank (New page: <pre> LOCUS 234.pME-H2A-mCherry 3651 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Par...) (current)
- 04:05, 19 December 2007 (diff | hist) . . (+6,842) . . N PME-nlsmCherry Genbank (New page: <pre> LOCUS 233.pME-nlsmCherry 3288 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Para...) (current)
- 04:05, 19 December 2007 (diff | hist) . . (+6,638) . . N PME-mCherry Genbank (New page: <pre> LOCUS 386.pME-mCherry 3261 bp ds-DNA circular 10-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant ...) (current)
- 04:04, 19 December 2007 (diff | hist) . . (+7,052) . . N PME-nlsEGFP Genbank (New page: <pre> LOCUS 385.pME-nlsEGFP 3342 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant ...) (current)
- 04:03, 19 December 2007 (diff | hist) . . (+7,080) . . N PME-EGFPCAAX Genbank (New page: <pre> LOCUS 384.pME-EGFPCAAX 3345 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant...) (current)
- 04:02, 19 December 2007 (diff | hist) . . (+7,047) . . N PME-EGFP Genbank (New page: <pre> LOCUS 383.pME-EGFP 3327 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant JM,...) (current)
- 04:02, 19 December 2007 (diff | hist) . . (+5,859) . . N P5E-Fse-Asc Genbank (New page: <pre> LOCUS 381.p5E-Fse-Asc 2663 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant ...) (current)
- 04:01, 19 December 2007 (diff | hist) . . (+1) . . m P5E-Fse-Asc (→Sequence) (current)
- 04:01, 19 December 2007 (diff | hist) . . (+6,530) . . N P5E-MCS Genbank (New page: <pre> LOCUS 228.p5E-MCS 2810 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant JM, ...) (current)
- 04:01, 19 December 2007 (diff | hist) . . (+7,318) . . N P5E-UAS Genbank (New page: <pre> LOCUS 327.p5E-UAS 3126 bp ds-DNA circular 10-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant JM, Yo...) (current)
- 03:59, 19 December 2007 (diff | hist) . . (+7,797) . . N P5E-hsp70l Genbank (New page: <pre> LOCUS 222.p5E-hsp70 4163 bp ds-DNA circular 09-DEC-2007 DEFINITION REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, ...)
- 03:59, 19 December 2007 (diff | hist) . . (+7,501) . . N P5E-CMV-SP6 Genbank (New page: <pre> LOCUS 382.p5E-CMV/SP6 3704 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant ...) (current)
- 03:57, 19 December 2007 (diff | hist) . . (+7,428) . . N P5E-h2afx Genbank (New page: <pre> LOCUS 380.p5E-h2afx 3604 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant JM...) (current)
- 03:55, 19 December 2007 (diff | hist) . . (+48) . . PDestTol2CG2 (current)
- 03:55, 19 December 2007 (diff | hist) . . (+48) . . PDestTol2pA2 (current)
- 03:54, 19 December 2007 (diff | hist) . . (+54) . . P3E-IRES-nlsEGFPpA
- 03:54, 19 December 2007 (diff | hist) . . (+55) . . P3E-IRES-EGFPCAAXpA
- 03:53, 19 December 2007 (diff | hist) . . (+51) . . P3E-IRES-EGFPpA
- 03:53, 19 December 2007 (diff | hist) . . (+49) . . P3E-mCherrypA (current)
- 03:52, 19 December 2007 (diff | hist) . . (+46) . . P3E-EGFPpA (current)
- 03:52, 19 December 2007 (diff | hist) . . (+44) . . P3E-MTpA (current)
- 03:51, 19 December 2007 (diff | hist) . . (+45) . . P3E-polyA (current)
- 03:49, 19 December 2007 (diff | hist) . . (+55) . . PME-mCherry no stop (current)
- 03:48, 19 December 2007 (diff | hist) . . (+52) . . PME-EGFP no stop (current)
- 03:48, 19 December 2007 (diff | hist) . . (+51) . . PME-mCherryCAAX
- 03:48, 19 December 2007 (diff | hist) . . (+43) . . PME-MCS
- 03:47, 19 December 2007 (diff | hist) . . (+48) . . PME-Gal4VP16
- 03:46, 19 December 2007 (diff | hist) . . (+50) . . PME-H2AmCherry
- 03:46, 19 December 2007 (diff | hist) . . (+50) . . PME-nlsmCherry (current)
- 03:46, 19 December 2007 (diff | hist) . . (+47) . . PME-mCherry (current)
- 03:45, 19 December 2007 (diff | hist) . . (+47) . . PME-nlsEGFP (current)
- 03:44, 19 December 2007 (diff | hist) . . (+48) . . PME-EGFPCAAX (current)
- 03:44, 19 December 2007 (diff | hist) . . (+44) . . PME-EGFP (current)
- 03:43, 19 December 2007 (diff | hist) . . (+46) . . P5E-Fse-Asc
- 03:43, 19 December 2007 (diff | hist) . . (+43) . . P5E-MCS (current)
- 03:43, 19 December 2007 (diff | hist) . . (+43) . . P5E-UAS (current)
- 03:42, 19 December 2007 (diff | hist) . . (+45) . . P5E-hsp70l
- 03:42, 19 December 2007 (diff | hist) . . (+47) . . P5E-CMV/SP6 (current)
- 03:41, 19 December 2007 (diff | hist) . . (+45) . . P5E-h2afx (current)
- 02:41, 19 December 2007 (diff | hist) . . (+13,669) . . N P5E-bactin2 Genbank (New page: <pre> LOCUS 299.p5E-bactin2 7950 bp ds-DNA circular 10-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant ...) (current)
- 02:30, 19 December 2007 (diff | hist) . . (0) . . m P5E-bactin2 (→Sequence)
- 02:30, 19 December 2007 (diff | hist) . . (+47) . . P5E-bactin2
- 03:40, 12 December 2007 (diff | hist) . . (0) . . Att site sequences (→list of att sites) (current)
- 20:21, 10 December 2007 (diff | hist) . . (+8) . . P3E-IRES-nlsEGFPpA (→Construction details)
- 20:18, 10 December 2007 (diff | hist) . . (+29) . . P3E-IRES-EGFPCAAXpA (→Construction details)
- 00:36, 10 December 2007 (diff | hist) . . (+163) . . Main Page (→Revision history)
- 00:34, 10 December 2007 (diff | hist) . . (+557) . . Att site sequences
- 00:31, 10 December 2007 (diff | hist) . . (-32) . . Att site sequences (→att site shared sequences)
- 12:11, 7 November 2007 (diff | hist) . . (+12) . . N MediaWiki:Pagetitle (New page: $1 - Tol2kit) (current)
- 04:13, 6 November 2007 (diff | hist) . . (+11) . . MediaWiki:Sidebar
- 04:13, 6 November 2007 (diff | hist) . . (-21) . . Protocols (→Assembling sequences for expression clones)
- 04:10, 6 November 2007 (diff | hist) . . (+567) . . Protocols
- 04:06, 6 November 2007 (diff | hist) . . (+60) . . Inserts and ends (→What are these sequences?) (current)
- 04:05, 6 November 2007 (diff | hist) . . (+135) . . Main Page (→Revision history)
- 04:03, 6 November 2007 (diff | hist) . . (+5) . . MediaWiki:Sidebar
- 04:03, 6 November 2007 (diff | hist) . . (+51) . . MediaWiki:Sidebar
- 04:01, 6 November 2007 (diff | hist) . . (+52,829) . . Inserts and ends
- 03:58, 6 November 2007 (diff | hist) . . (+45) . . N File:Expression clone.png (example of expression clone sequence assembly) (current)
- 03:57, 6 November 2007 (diff | hist) . . (+480) . . Inserts and ends
- 03:53, 6 November 2007 (diff | hist) . . (+1,136) . . N Inserts and ends (New page: Below are insert sequences for all entry clones, and 5' and 3' end sequences for all destination clones. In all cases, we have included sequence up through the appropriate "att shared" seq...)
- 03:40, 6 November 2007 (diff | hist) . . (+1) . . m List of entry and destination vectors
- 03:40, 6 November 2007 (diff | hist) . . (+58) . . List of entry and destination vectors
- 03:39, 6 November 2007 (diff | hist) . . (+137) . . List of entry and destination vectors
- 02:06, 6 November 2007 (diff | hist) . . (+47) . . List of entry and destination vectors
- 23:19, 2 November 2007 (diff | hist) . . (+109) . . PME-mCherry no stop
- 23:19, 2 November 2007 (diff | hist) . . (+101) . . Main Page (→Clone distribution)
- 23:18, 2 November 2007 (diff | hist) . . (+2) . . Main Page (→'''The Tol2kit''')
- 23:17, 2 November 2007 (diff | hist) . . (+128) . . Main Page (→Revision history)
- 23:15, 2 November 2007 (diff | hist) . . (+62) . . N File:PME-mCherry no stop.png (Sequencher snapshot showing components of pME-mCherry no stop.) (current)
- 23:15, 2 November 2007 (diff | hist) . . (+9,404) . . N PME-mCherry no stop sequence (New page: <pre> >pME-mCherry-no_stop CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGC...) (current)
- 23:14, 2 November 2007 (diff | hist) . . (+492) . . N PME-mCherry no stop (New page: === Construction details === pME-mCherry no stop was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases ...)
- 23:13, 2 November 2007 (diff | hist) . . (+61) . . N File:PME-EGFP no stop.png (Sequencher screenshot showing components of pME-EGFP no stop.) (current)
- 23:12, 2 November 2007 (diff | hist) . . (+9,625) . . N PME-EGFP no stop sequence (New page: <pre> >pME-EGFP_no_stop CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGA...) (current)
- 23:11, 2 November 2007 (diff | hist) . . (+623) . . N PME-EGFP no stop (New page: === Construction details === pME-EGFP no stop was made by PCR amplification of the EGFP insert from a pCS2-based plasmid, using primers that add att sites, followed by a BP reaction. Sequ...)
- 23:09, 2 November 2007 (diff | hist) . . (+6,958) . . PME-mCherryCAAX sequence H80D (current)
- 23:07, 2 November 2007 (diff | hist) . . (+719) . . PME-mCherryCAAX
- 23:03, 2 November 2007 (diff | hist) . . (+65) . . N File:PME-mCherryCAAX H80D.png (Sequencher screenshot showing components of pME-mCherryCAAX H80D.) (current)
- 23:03, 2 November 2007 (diff | hist) . . (+5) . . PME-mCherryCAAX H80D (→Crude map) (current)
- 23:00, 2 November 2007 (diff | hist) . . (0) . . PME-mCherryCAAX H80D (→Crudely annotated sequence)
- 23:00, 2 November 2007 (diff | hist) . . (0) . . m PME-mCherryCAAX H80D sequence (PME-mCherryCAAX sequence H80D moved to PME-mCherryCAAX H80D sequence: indicating H80D mutation in page name) (current)
- 23:00, 2 November 2007 (diff | hist) . . (+43) . . N PME-mCherryCAAX sequence H80D (PME-mCherryCAAX sequence H80D moved to PME-mCherryCAAX H80D sequence: indicating H80D mutation in page name)
- 23:00, 2 November 2007 (diff | hist) . . (+5) . . PME-mCherryCAAX H80D (→Crudely annotated sequence)
- 22:59, 2 November 2007 (diff | hist) . . (0) . . m PME-mCherryCAAX H80D sequence (PME-mCherryCAAX sequence moved to PME-mCherryCAAX sequence H80D: indicating H80D mutation in page name)
- 22:59, 2 November 2007 (diff | hist) . . (+43) . . N PME-mCherryCAAX sequence (PME-mCherryCAAX sequence moved to PME-mCherryCAAX sequence H80D: indicating H80D mutation in page name) (current)
- 22:59, 2 November 2007 (diff | hist) . . (-14) . . PME-mCherryCAAX H80D
- 22:57, 2 November 2007 (diff | hist) . . (+820) . . List of entry and destination vectors
- 22:53, 2 November 2007 (diff | hist) . . (0) . . m PME-mCherryCAAX H80D (PME-mCherryCAAX moved to PME-mCherryCAAX H80D: adding page for pME-mCherryCAAX without mutation)
- 22:53, 2 November 2007 (diff | hist) . . (+34) . . N PME-mCherryCAAX (PME-mCherryCAAX moved to PME-mCherryCAAX H80D: adding page for pME-mCherryCAAX without mutation)
- 11:34, 31 October 2007 (diff | hist) . . (+51) . . MediaWiki:Sidebar
- 11:33, 31 October 2007 (diff | hist) . . (+248) . . Sources (→References) (current)
- 11:32, 31 October 2007 (diff | hist) . . (+5) . . Sources (→Transposon backbone)
- 11:24, 31 October 2007 (diff | hist) . . (+150) . . Sources
- 11:20, 31 October 2007 (diff | hist) . . (+52) . . Main Page (→Links)
- 17:14, 30 October 2007 (diff | hist) . . (0) . . Main Page (→Citing the Tol2kit)
- 17:12, 30 October 2007 (diff | hist) . . (+39) . . Main Page (→Citing the Tol2kit)
- 12:28, 12 October 2007 (diff | hist) . . (+144) . . Invitrogen manual
- 03:35, 12 October 2007 (diff | hist) . . (+4) . . m Main Page (→Revision history)
- 03:35, 12 October 2007 (diff | hist) . . (-65) . . Main Page (→About this site)
- 19:06, 11 October 2007 (diff | hist) . . (+141) . . Main Page
- 19:03, 11 October 2007 (diff | hist) . . (-85) . . Protocols
- 19:01, 11 October 2007 (diff | hist) . . (-41) . . Protocols
- 19:01, 11 October 2007 (diff | hist) . . (+844) . . N Invitrogen manual (link to archived copy of Invitrogen manual)
- 18:54, 11 October 2007 (diff | hist) . . (-10) . . Protocols (→Donor Vectors)
- 18:54, 11 October 2007 (diff | hist) . . (-11) . . Protocols (→What you will need)
- 18:53, 11 October 2007 (diff | hist) . . (-10) . . Protocols
- 18:51, 11 October 2007 (diff | hist) . . (0) . . Main Page
- 02:22, 29 September 2007 (diff | hist) . . (+352) . . P5E-MCS (→Construction details)
- 02:14, 29 September 2007 (diff | hist) . . (+186) . . PME-MCS (→Construction details)
- 02:14, 28 September 2007 (diff | hist) . . (+3) . . List of entry and destination vectors
- 02:06, 28 September 2007 (diff | hist) . . (+52) . . N File:PME-MCS.png (Sequencher screenshot showing components of pME-MCS.) (current)
- 02:02, 28 September 2007 (diff | hist) . . (+7,945) . . N PME-MCS sequence (New page: <pre> >pME-MCS CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTAAT...) (current)
- 23:32, 27 September 2007 (diff | hist) . . (+167) . . PME-MCS (→Construction details)
- 23:30, 27 September 2007 (diff | hist) . . (+794) . . N PME-MCS (New page: === Construction details === pME-MCS was made by PCR of the pBSII SK+ multiple cloning site, from M13F to M13R primers, followed by a BP reaction with pDONR221. Sequencing confirms that al...)
- 03:21, 24 September 2007 (diff | hist) . . (+301) . . Main Page (→Revision history)
- 03:12, 24 September 2007 (diff | hist) . . (+573) . . Sources
- 13:51, 15 September 2007 (diff | hist) . . (+447) . . N Invitrogen ccdB (New page: ccdB sequence found in pDest R4-R3. The lowercase "c" indicates a T>C mutation relative to the ccdB sequence from Genbank. <pre> ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATG...) (current)
- 13:47, 15 September 2007 (diff | hist) . . (+1,091) . . Sources
- 13:36, 15 September 2007 (diff | hist) . . (+214) . . Basic Gateway principles (current)
- 13:29, 15 September 2007 (diff | hist) . . (-17) . . Main Page (→About this site)
- 13:28, 15 September 2007 (diff | hist) . . (0) . . Main Page (→About this site)
- 13:27, 15 September 2007 (diff | hist) . . (+21) . . N Components used for the Tol2kit (Components used for the Tol2kit moved to Sources: combining with References page, renaming appropriately) (current)
- 13:27, 15 September 2007 (diff | hist) . . (0) . . m Sources (Components used for the Tol2kit moved to Sources: combining with References page, renaming appropriately)
- 12:42, 14 September 2007 (diff | hist) . . (+82) . . List of entry and destination vectors
- 16:17, 12 September 2007 (diff | hist) . . (+11) . . m Main Page (→Citing the Tol2kit)
- 16:17, 12 September 2007 (diff | hist) . . (+62) . . Main Page (→Citing the Tol2kit)
- 11:58, 12 September 2007 (diff | hist) . . (-49) . . Main Page (→About this site)
- 13:17, 11 September 2007 (diff | hist) . . (+9) . . Main Page (→Citing the Tol2kit)
- 13:16, 11 September 2007 (diff | hist) . . (+35) . . Sample results with the Tol2kit (current)
- 13:08, 11 September 2007 (diff | hist) . . (-18) . . Basic Gateway principles
- 13:07, 11 September 2007 (diff | hist) . . (+1,322) . . Basic Gateway principles (→How we use this in the Tol2kit)
- 12:58, 11 September 2007 (diff | hist) . . (-46) . . Main Page (→About this site)
- 12:57, 11 September 2007 (diff | hist) . . (-2) . . Main Page (→'''The Tol2kit''')
- 20:53, 17 August 2007 (diff | hist) . . (+242) . . Main Page (→Revision history)
- 20:49, 17 August 2007 (diff | hist) . . (+46) . . MediaWiki:Sidebar
- 22:53, 25 June 2007 (diff | hist) . . (+19) . . PME-mCherryCAAX H80D (→Construction details)
- 22:52, 25 June 2007 (diff | hist) . . (+131) . . PME-mCherryCAAX H80D (→Construction details)
- 22:50, 25 June 2007 (diff | hist) . . (+5) . . PME-mCherryCAAX H80D sequence
- 03:04, 21 June 2007 (diff | hist) . . (+562) . . PDestTol2pA (→Construction details) (current)
- 20:51, 18 June 2007 (diff | hist) . . (+577) . . PME-mCherryCAAX H80D (→Construction details)
- 18:08, 7 June 2007 (diff | hist) . . (+4) . . m Main Page (→Revision history)
- 18:08, 7 June 2007 (diff | hist) . . (+77) . . m Main Page (→Revision history)
- 18:07, 7 June 2007 (diff | hist) . . (+392) . . List of entry and destination vectors
- 17:51, 7 June 2007 (diff | hist) . . (+200) . . Main Page (→Revision history)
- 17:50, 7 June 2007 (diff | hist) . . (+6) . . PDestTol2CG2 sequence (current)
- 17:49, 7 June 2007 (diff | hist) . . (+69) . . PDestTol2CG2 (→Crudely annotated sequence)
- 17:48, 7 June 2007 (diff | hist) . . (+6) . . PDestTol2pA2 sequence (current)
- 17:47, 7 June 2007 (diff | hist) . . (+69) . . PDestTol2pA2 (→Crudely annotated sequence)
- 17:44, 7 June 2007 (diff | hist) . . (+6) . . PDestTol2CG sequence (current)
- 17:43, 7 June 2007 (diff | hist) . . (+69) . . PDestTol2CG (→Crudely annotated sequence) (current)
- 17:42, 7 June 2007 (diff | hist) . . (+7) . . PDestTol2pA sequence (current)
- 17:41, 7 June 2007 (diff | hist) . . (+69) . . PDestTol2pA (→Crudely annotated sequence)
- 16:05, 5 June 2007 (diff | hist) . . (+8) . . Sample results with the Tol2kit (→Validation of IRES clones)
- 16:01, 5 June 2007 (diff | hist) . . (+95) . . N File:IRES results.jpg (Confocal images of trunk of 24 hpf embryos mosaically expressing various IRES-based constructs.) (current)
- 16:00, 5 June 2007 (diff | hist) . . (+358) . . Main Page
- 15:55, 5 June 2007 (diff | hist) . . (+1,143) . . Sample results with the Tol2kit
- 03:08, 17 May 2007 (diff | hist) . . (+325) . . Protocols
- 22:04, 13 May 2007 (diff | hist) . . (-33) . . P5E-UAS (→Construction details)
- 16:17, 11 May 2007 (diff | hist) . . (+120) . . m Protocols (→LR reactions)
- 16:05, 11 May 2007 (diff | hist) . . (+173) . . Main Page
- 12:03, 11 May 2007 (diff | hist) . . (-1) . . PDestTol2pA2 (→Construction details)
- 12:03, 11 May 2007 (diff | hist) . . (-1) . . PDestTol2CG2 (→Construction details)
- 02:02, 2 May 2007 (diff | hist) . . (-194) . . m Main Page (→Revision history)
- 02:01, 2 May 2007 (diff | hist) . . (+126) . . Main Page
- 01:46, 2 May 2007 (diff | hist) . . (-33) . . MediaWiki:Sidebar
- 01:45, 2 May 2007 (diff | hist) . . (-7) . . MediaWiki:Sidebar
- 01:45, 2 May 2007 (diff | hist) . . (-7) . . Main Page
- 01:42, 2 May 2007 (diff | hist) . . (0) . . MediaWiki:Sidebar
- 01:41, 2 May 2007 (diff | hist) . . (+1) . . MediaWiki:Sidebar
- 01:40, 2 May 2007 (diff | hist) . . (+7) . . MediaWiki:Sidebar
- 01:29, 2 May 2007 (diff | hist) . . (+32) . . MediaWiki:Sidebar
- 01:18, 2 May 2007 (diff | hist) . . (-417) . . List of entry and destination vectors
- 01:17, 2 May 2007 (diff | hist) . . (+474) . . Main Page
- 01:09, 2 May 2007 (diff | hist) . . (+1) . . m List of entry and destination vectors (→Revision history)
- 00:44, 2 May 2007 (diff | hist) . . (+62) . . MediaWiki:Sidebar
- 17:04, 30 April 2007 (diff | hist) . . (+120) . . P3E-mCherrypA (→Construction details)
- 17:03, 30 April 2007 (diff | hist) . . (+162) . . P3E-EGFPpA (→Construction details)
- 13:54, 22 April 2007 (diff | hist) . . (+43) . . MediaWiki:Sidebar
- 13:50, 22 April 2007 (diff | hist) . . (+59) . . MediaWiki:Sidebar
- 13:48, 22 April 2007 (diff | hist) . . (+163) . . Main Page (→Clone distribution)
- 21:37, 18 April 2007 (diff | hist) . . (+14) . . MediaWiki:Sidebar
- 21:36, 18 April 2007 (diff | hist) . . (+233) . . N MediaWiki:Sidebar (New page: * navigation ** mainpage|mainpage ** List of entry and destination vectors|Entry/Dest clone list ** portal-url|portal ** currentevents-url|currentevents ** recentchanges-url|recentchanges ...)
- 21:35, 18 April 2007 (diff | hist) . . (+37) . . Main Page (→About this site)
- 05:07, 18 April 2007 (diff | hist) . . (+56) . . N File:PDestTol2CG.png (Sequencher screenshot showing components of pDestTol2CG.) (current)
- 05:06, 18 April 2007 (diff | hist) . . (+21,422) . . N PDestTol2CG sequence (New page: <pre> >pDestTol2CG CACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAA TACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGC CCTTATTCCCTTTTTT...)
- 05:05, 18 April 2007 (diff | hist) . . (+604) . . N PDestTol2CG (New page: === Construction details === pDestTol2CG was made by PCRing the cmlc2:egfp-pA cassette, adding BglII sites in the primers, then cloning into the unique BglII site of pDestTol2pA and select...)
- 05:01, 18 April 2007 (diff | hist) . . (+18,833) . . PDestTol2pA sequence
- 04:56, 18 April 2007 (diff | hist) . . (+56) . . N File:PDestTol2pA.png (Sequencher screenshot showing components of pDestTol2pA.) (current)
- 04:54, 18 April 2007 (diff | hist) . . (+395) . . N PDestTol2pA (New page: === Construction details === pDestTol2pA was built by Clemens Grabher; we are in the process of asking him for construction details. === Crudely annotated sequence === FASTA file with the...)
- 04:53, 18 April 2007 (diff | hist) . . (+148) . . List of entry and destination vectors
- 02:51, 30 March 2007 (diff | hist) . . (+2) . . m Main Page (→Clone distribution)
- 03:34, 24 March 2007 (diff | hist) . . (+9) . . m PCS2FA-transposase (→Construction details)
- 03:33, 24 March 2007 (diff | hist) . . (+63) . . N File:PCS2FA-transposase.png (Sequencher screenshot showing components of pCS2FA-transposase.) (current)
- 03:32, 24 March 2007 (diff | hist) . . (+11,476) . . N PCS2FA-transposase sequence (New page: <pre> >pCS2FA-transposase TCGACCATAGCCAATTCAATATGGCGTATATGGACTCATGCCAATTCAAT ATGGTGGATCTGGACCTGTGCCAATTCAATATGGCGTATATGGACTCGTG CCAATTCAATATGGTGGATCTGGACCCCAGCCAATTCAATATGGCGGACT TGGCACCAT...) (current)
- 03:31, 24 March 2007 (diff | hist) . . (+748) . . N PCS2FA-transposase (New page: === Construction details === pCS2FA-transposase was made by PCRing the Tol2 transpoase cds from the original Kawakami clone, adding restriction sites, then conventionally subcloned into pC...)
- 03:26, 24 March 2007 (diff | hist) . . (-10) . . List of entry and destination vectors
- 03:24, 24 March 2007 (diff | hist) . . (+99) . . List of entry and destination vectors
- 03:12, 24 March 2007 (diff | hist) . . (+58) . . N File:P3E-mCherrypA.png (Sequencher screenshot showing components of p3E-mCherrypA.) (current)
- 03:11, 24 March 2007 (diff | hist) . . (+7,642) . . N P3E-mCherrypA sequence (New page: <pre> >p3E-mCherrypA CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTC...) (current)
- 03:11, 24 March 2007 (diff | hist) . . (+672) . . N P3E-mCherrypA (New page: === Construction details === p3E-mCherrypA was made by PCR amplification of mCherry and an SV40 late polyadenylation signal from a pCS2+-based construct, using primers to add att sites, fo...)
- 03:07, 24 March 2007 (diff | hist) . . (+55) . . N File:P3E-EGFPpA.png (Sequencher screenshot showing components of p3E-EGFPpA.) (current)
- 03:07, 24 March 2007 (diff | hist) . . (+32) . . P3E-EGFPpA (→Construction details)
- 03:06, 24 March 2007 (diff | hist) . . (+7,836) . . N P3E-EGFPpA sequence (New page: <pre> >p3E_EGFPpA CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATT...) (current)
- 03:05, 24 March 2007 (diff | hist) . . (+714) . . N P3E-EGFPpA (New page: === Construction details === p3E-EGFPpA was made by PCR amplification of EGFP and an SV40 late polyadenylation signal from a pCS2+-based plasmid, using primers to add att sites, followed b...)
- 03:03, 24 March 2007 (diff | hist) . . (+5) . . m P3E-MTpA (→Construction details)
- 03:02, 24 March 2007 (diff | hist) . . (+53) . . N File:P3E-MTpA.png (Sequencher screenshot showing components of p3E-MTpA.) (current)
- 03:02, 24 March 2007 (diff | hist) . . (0) . . m P3E-MTpA (→Construction details)
- 03:01, 24 March 2007 (diff | hist) . . (+6,300) . . N P3E-MTpA sequence (New page: <pre> >p3E_MT-pA CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTA...) (current)
- 03:00, 24 March 2007 (diff | hist) . . (+507) . . N P3E-MTpA (New page: === Construction details === p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 polyadenylation signal from pCS2-MT, using primers to add att sequences, followed by a BP reaction....)
- 02:35, 24 March 2007 (diff | hist) . . (0) . . m P3E-polyA (→Construction details)
- 02:34, 24 March 2007 (diff | hist) . . (+54) . . N File:P3E-polyA.png (Sequencher screenshot showing components of p3E-polyA.) (current)
- 02:32, 24 March 2007 (diff | hist) . . (+6) . . P3E-polyA sequence (current)
- 02:32, 24 March 2007 (diff | hist) . . (+5,363) . . N P3E-polyA sequence (New page: <pre> >p3E-polyA CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTA...)
- 02:31, 24 March 2007 (diff | hist) . . (+501) . . N P3E-polyA (New page: === Construction details === p3E-polyA was made by PCR amplificaiton of the SV40 late polyadenylation signal from pCS2+, using primers that add att sites, followed by a BP reaction. Sequen...)
- 02:18, 24 March 2007 (diff | hist) . . (+57) . . N File:PME-Gal4VP16.png (Sequencher screenshot showing components of pME-Gal4VP16.) (current)
- 02:18, 24 March 2007 (diff | hist) . . (+63) . . m PME-Gal4VP16 (→Construction details)
- 02:16, 24 March 2007 (diff | hist) . . (+6,586) . . N PME-Gal4VP16 sequence (New page: <pre> >pME_Gal4VP16 CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCA...) (current)
- 02:16, 24 March 2007 (diff | hist) . . (+460) . . N PME-Gal4VP16 (New page: === Construction details === pME-Gal4VP16 was made by PCR amplification of Gal4VP16, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation,...)
- 02:14, 24 March 2007 (diff | hist) . . (+59) . . N File:PME-H2AmCherry.png (Sequencher screenshot showing components of pME-H2AmCherry.) (current)
- 02:13, 24 March 2007 (diff | hist) . . (+7,973) . . N PME-H2AmCherry sequence (New page: <pre> >pME-H2AF/Z-mCherry CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCC...) (current)
- 02:13, 24 March 2007 (diff | hist) . . (+520) . . N PME-H2AmCherry (New page: === Construction details === pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR) to the N terminus of mCherry, using primers that add att sites, followed by a...)
- 02:06, 24 March 2007 (diff | hist) . . (+59) . . N File:PME-nlsmCherry.png (Sequencher screenshot showing components of pME-nlsmCherry.) (current)
- 02:06, 24 March 2007 (diff | hist) . . (+6,864) . . N PME-nlsmCherry sequence (New page: <pre> >pME-nlsmCherry CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATT...) (current)
- 02:04, 24 March 2007 (diff | hist) . . (+142) . . PME-nlsmCherry
- 02:02, 24 March 2007 (diff | hist) . . (+536) . . N PME-nlsmCherry (New page: === Construction details === pME-nlsmCherry was made by PCR amplification of an nls-mCherry (mCherry with nuclear-localization signal) cassette, using primers that add att sites, followed ...)
- 01:59, 24 March 2007 (diff | hist) . . (+60) . . N File:PME-mCherryCAAX.png (Sequencher screenshot showing components of pME-mCherryCAAX.) (current)
- 01:58, 24 March 2007 (diff | hist) . . (+7,001) . . N PME-mCherryCAAX H80D sequence (New page: <pre> >pME-mCherryCAAX CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGAT...)
- 01:57, 24 March 2007 (diff | hist) . . (+601) . . N PME-mCherryCAAX H80D (New page: === Construction details === pME-mCherryCAAX was made by PCR amplification of mCherry-CAAX, using primers to add att sites, followed by a BP reaction. mCherry-CAAX is mCherry with a C-term...)
- 01:54, 24 March 2007 (diff | hist) . . (+56) . . N File:PME-mCherry.png (Sequencher screenshot showing components of pME-mCherry.) (current)
- 01:53, 24 March 2007 (diff | hist) . . (+6,771) . . N PME-mCherry sequence (New page: <pre> >pME-mCherry CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCAT...) (current)
- 01:52, 24 March 2007 (diff | hist) . . (+468) . . N PME-mCherry (New page: === Construction details === pME-mCherry was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the i...)
- 01:51, 24 March 2007 (diff | hist) . . (+16) . . m PME-nlsEGFP (→Construction details)
- 01:50, 24 March 2007 (diff | hist) . . (+56) . . N File:PME-nlsEGFP.png (Sequencher screenshot showing components of pME-nlsEGFP.) (current)
- 01:49, 24 March 2007 (diff | hist) . . (+6,970) . . N PME-nlsEGFP sequence (New page: <pre> >pME-nlsEGFP CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCAT...) (current)
- 01:48, 24 March 2007 (diff | hist) . . (+505) . . N PME-nlsEGFP (New page: === Construction details === pME-nlsEGFP was made by PCR amplification of an nls-EGFP (nuclear-localized GFP) cassette, using primers that add att sites, followed by a BP reaction. Sequen...)
- 01:46, 24 March 2007 (diff | hist) . . (+57) . . N File:PME-EGFPCAAX.png (Sequencher screenshot showing components of pME-EGFPCAAX.) (current)
- 01:45, 24 March 2007 (diff | hist) . . (+7,079) . . N PME-EGFPCAAX sequence (New page: <pre> >pME-EGFP-CAAX CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTC...) (current)
- 01:44, 24 March 2007 (diff | hist) . . (+583) . . N PME-EGFPCAAX (New page: === Construction details === pME-EGFPCAAX was made by PCR amplification of EGFP-CAAX, using primers to add att sites, followed by a BP reaction. EGFP-CAAX is EGFP with a C-terminal fusion ...)
- 01:40, 24 March 2007 (diff | hist) . . (+37) . . m PME-EGFP (→Construction details)
- 01:39, 24 March 2007 (diff | hist) . . (+1) . . PME-EGFP (→Construction details)
- 01:38, 24 March 2007 (diff | hist) . . (+186) . . PME-EGFP (→Construction details)
- 01:35, 24 March 2007 (diff | hist) . . (+53) . . N File:PME-EGFP.png (Sequencher screenshot showing components of pME-EGFP.) (current)
- 01:34, 24 March 2007 (diff | hist) . . (+7,002) . . N PME-EGFP sequence (New page: <pre> >pME-EGFP CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTAA...) (current)
- 01:32, 24 March 2007 (diff | hist) . . (+456) . . N PME-EGFP (New page: === Construction details === pME-EGFP was made by PCR amplification of EGFP, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert ...)
- 01:27, 24 March 2007 (diff | hist) . . (+52) . . List of entry and destination vectors
- 03:53, 23 March 2007 (diff | hist) . . (+41) . . P5E-UAS (→Construction details)
- 03:52, 23 March 2007 (diff | hist) . . (+52) . . N File:P5E-UAS.png (Sequencher screenshot showing components of p5E-UAS.) (current)
- 03:51, 23 March 2007 (diff | hist) . . (+6,169) . . N P5E-UAS sequence (New page: <pre> >p5E_UAS CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTAAT...)
- 03:51, 23 March 2007 (diff | hist) . . (+997) . . N P5E-UAS (New page: === Construction details === p5E-UAS was made by conventionally subcloning into p5E-MCS a HindIII-EcoRI fragment from a UAS-E1b construct from Reinhard Köster, containing multimerized Gal...)
- 03:33, 23 March 2007 (diff | hist) . . (+19) . . m P5E-hsp70l (→Construction details)
- 03:32, 23 March 2007 (diff | hist) . . (+58) . . N File:P5E-hsp70.png (Sequencher screenshot showing components of p5E-''hsp70''.) (current)
- 03:31, 23 March 2007 (diff | hist) . . (+9,424) . . N P5E-hsp70l sequence (New page: <pre> >p5E-hsp70 CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTA...)
- 03:31, 23 March 2007 (diff | hist) . . (+597) . . N P5E-hsp70l (New page: === Construction details === p5E-''hsp70'' was made by PCR amplification of the 1.5 kb hsp70 promoter, using primers to add att sites, followed by a BP reaction. Sequencing shows a single...)
- 03:24, 23 March 2007 (diff | hist) . . (+56) . . N File:P5E-CMV-SP6.png (Sequencher screenshot showing components of p5E-CMV/SP6.) (current)
- 03:23, 23 March 2007 (diff | hist) . . (+7,957) . . N P5E-CMV-SP6 sequence (New page: <pre> >p5E-CMV-SP6 CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCAT...) (current)
- 03:22, 23 March 2007 (diff | hist) . . (+821) . . N P5E-CMV/SP6 (New page: === Construction details === p5E-CMV/SP6 was made by PCR amplification of the sCMV and SP6 promoters from pCS2+, using primers that added att sites, followed by a BP reaction. This 5' el...)
- 22:29, 22 March 2007 (diff | hist) . . (+58) . . N File:P5E-h2afx.png (Sequencher screenshot showing components of p5E-''h2afx''.) (current)
- 22:28, 22 March 2007 (diff | hist) . . (+7,753) . . N P5E-h2afx sequence (New page: <pre> >p5E-h2afx CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTA...) (current)
- 22:27, 22 March 2007 (diff | hist) . . (+1,008) . . N P5E-h2afx (New page: === Construction details === p5E-''h2afx'' was made by PCR amplification of 1 kb genomic DNA immediately upstream of the start codon of ''h2afx'' (NM_201073), using primers that added att ...)
- 22:09, 22 March 2007 (diff | hist) . . (+36) . . List of entry and destination vectors
- 21:35, 22 March 2007 (diff | hist) . . (+822) . . Main Page
- 23:20, 19 March 2007 (diff | hist) . . (+371) . . Protocols
- 23:16, 19 March 2007 (diff | hist) . . (+82) . . List of entry and destination vectors
- 23:14, 19 March 2007 (diff | hist) . . (+152) . . P5E-MCS sequence (current)
- 23:13, 19 March 2007 (diff | hist) . . (-2,735) . . P5E-Fse-Asc sequence (current)
- 23:12, 19 March 2007 (diff | hist) . . (-2,731) . . P3E-IRES-EGFPpA sequence (current)
- 23:11, 19 March 2007 (diff | hist) . . (-3,565) . . P3E-IRES-EGFPCAAXpA sequence (current)
- 23:10, 19 March 2007 (diff | hist) . . (-2,738) . . P3E-IRES-nlsEGFPpA sequence (current)
- 23:08, 19 March 2007 (diff | hist) . . (-1,362) . . PDestTol2pA2 sequence
- 23:05, 19 March 2007 (diff | hist) . . (-1,393) . . PDestTol2CG2 sequence
- 23:00, 19 March 2007 (diff | hist) . . (-6) . . m PDestTol2CG2
- 22:59, 19 March 2007 (diff | hist) . . (-6) . . m PDestTol2pA2
- 22:58, 19 March 2007 (diff | hist) . . (-6) . . m P3E-IRES-nlsEGFPpA
- 22:57, 19 March 2007 (diff | hist) . . (-6) . . m P3E-IRES-EGFPCAAXpA
- 22:57, 19 March 2007 (diff | hist) . . (-6) . . m P3E-IRES-EGFPpA
- 22:56, 19 March 2007 (diff | hist) . . (-6) . . m P5E-Fse-Asc
- 22:55, 19 March 2007 (diff | hist) . . (-6) . . P5E-MCS
- 22:53, 19 March 2007 (diff | hist) . . (+56) . . N File:P5E-bactin2.png (Sequencher screenshot showing components of p5E-bactin2.) (current)
- 22:52, 19 March 2007 (diff | hist) . . (+22,557) . . N P5E-bactin2 sequence (New page: <pre> >p5E-bactin2 CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCAT...) (current)
- 21:00, 19 March 2007 (diff | hist) . . (+1,098) . . N P5E-bactin2 (New page: === Construction details === ''bactin2'' genomic sequence was derived from a 5.3 kb clone (gift of Ken Poss), which was derived in turn from a 10 kb upstream construct from Shin-ichi Higas...)
- 20:51, 19 March 2007 (diff | hist) . . (+16) . . List of entry and destination vectors
- 14:34, 24 February 2007 (diff | hist) . . (+3,536) . . N Att site sequences (New page: == att site shared sequences == Here is a set of "shared" sequences--the core stretch that is shared between attB/P/L/R for att1-4. These are useful for stitching together entry and destin...)
- 14:21, 24 February 2007 (diff | hist) . . (+39) . . m Protocols (→What you will need)
- 14:58, 23 February 2007 (diff | hist) . . (-12) . . m Protocols (→BP Reactions)
- 13:20, 23 February 2007 (diff | hist) . . (+300) . . Protocols
- 13:12, 23 February 2007 (diff | hist) . . (+259) . . Protocols
- 17:10, 21 February 2007 (diff | hist) . . (-56) . . m Protocols (→What you will need)
- 20:42, 20 February 2007 (diff | hist) . . (+229) . . List of entry and destination vectors
- 19:29, 20 February 2007 (diff | hist) . . (+192) . . Protocols (→What you will need)
- 19:27, 20 February 2007 (diff | hist) . . (+70) . . Protocols (→LR reactions)
- 19:19, 20 February 2007 (diff | hist) . . (+3) . . Protocols (→BP Reactions)
- 19:18, 20 February 2007 (diff | hist) . . (+399) . . Protocols (→BP Reactions)
- 19:10, 20 February 2007 (diff | hist) . . (+245) . . Protocols (→What you will need)
- 12:39, 16 February 2007 (diff | hist) . . (+161) . . Protocols
- 00:28, 13 February 2007 (diff | hist) . . (+97) . . Protocols
- 00:20, 13 February 2007 (diff | hist) . . (-2) . . m Protocols (→Growing up clones)
- 00:18, 13 February 2007 (diff | hist) . . (+990) . . Protocols
- 00:03, 13 February 2007 (diff | hist) . . (+56) . . N File:P5E-Fse-Asc.png (Sequencher screenshot showing components of p5E-Fse-Asc.) (current)
- 00:02, 13 February 2007 (diff | hist) . . (+7,528) . . N P5E-Fse-Asc sequence (New page: <pre> >p5E-Fse-Asc CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCAT...)
- 00:01, 13 February 2007 (diff | hist) . . (+616) . . N P5E-Fse-Asc (New page: '''Construction details'''<br> p5E-Fse-Asc was made by PCR of two overlapping primers with att sites and the Fse and Asc recognition sites, followed by a BP reaction. The insert consists o...)
- 23:56, 12 February 2007 (diff | hist) . . (+52) . . N File:P5E-MCS.png (Sequencher screenshot showing components of p5E-MCS.) (current)
- 23:55, 12 February 2007 (diff | hist) . . (+464) . . N P5E-MCS (New page: '''Construction details'''<br> p5E-MCS was made by PCR of the pBSII SK+ multiple cloning site, from T7 to T3 primers, followed by a BP reaction. Sequencing confirms that all bases of the i...)
- 23:51, 12 February 2007 (diff | hist) . . (+5,187) . . N P5E-MCS sequence (New page: <pre> >p5E_MCS CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGATTCATTAAT...)
- 23:46, 12 February 2007 (diff | hist) . . (+8) . . List of entry and destination vectors
- 19:44, 7 February 2007 (diff | hist) . . (+63) . . N File:P3E-IRES-nlsEGFPpA.png (Sequencher screenshot showing components of p3E-IRES-nlsEGFPpA.) (current)
- 19:43, 7 February 2007 (diff | hist) . . (-1) . . m P3E-IRES-nlsEGFPpA
- 19:43, 7 February 2007 (diff | hist) . . (+12,404) . . N P3E-IRES-nlsEGFPpA sequence (New page: <pre> >p3E-IRES-nlsEGFPpA CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCC...)
- 19:42, 7 February 2007 (diff | hist) . . (+662) . . N P3E-IRES-nlsEGFPpA (New page: '''Construction details'''<br> p3E-IRES-nlsEGFPpA was made by fusion PCR of the EMCV IRES sequence, and nlsEGFP-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all bas...)
- 19:40, 7 February 2007 (diff | hist) . . (+1) . . m P3E-IRES-EGFPCAAXpA
- 19:39, 7 February 2007 (diff | hist) . . (+64) . . N File:P3E-IRES-EGFPCAAXpA.png (Sequencher screenshot showing components of p3E-IRES-EGFPCAAXpA.) (current)
- 19:39, 7 February 2007 (diff | hist) . . (+13,299) . . N P3E-IRES-EGFPCAAXpA sequence (New page: <pre> >p3E-IRES-EGFPCAAXpA CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGC...)
- 19:38, 7 February 2007 (diff | hist) . . (+500) . . N P3E-IRES-EGFPCAAXpA (New page: ''Construction details'''<br> p3E-IRES-EGFPCAAXpA was made by fusion PCR of the EMCV IRES sequence, and EGFPCAAX-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all ba...)
- 19:31, 7 February 2007 (diff | hist) . . (+186) . . P3E-IRES-EGFPpA
- 19:30, 7 February 2007 (diff | hist) . . (+94) . . P3E-IRES-EGFPpA sequence
- 19:25, 7 February 2007 (diff | hist) . . (+60) . . N File:P3E-IRES-EGFPpA.png (Sequencher screenshot showing components of p3E-IRES-EGFPpA.) (current)
- 19:24, 7 February 2007 (diff | hist) . . (+12,308) . . N P3E-IRES-EGFPpA sequence (New page: <pre> >p3E-IRES-EGFPpA CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC GCGTTGGCCGAT...)
- 19:21, 7 February 2007 (diff | hist) . . (+486) . . N P3E-IRES-EGFPpA (New page: '''Construction details'''<br> p3E-IRES-EGFPpA was made by fusion PCR of the EMCV IRES sequence, and EGFP-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all bases of ...)
- 19:17, 7 February 2007 (diff | hist) . . (+1) . . File:PDestTol2pA2.png (current)
- 19:16, 7 February 2007 (diff | hist) . . (+57) . . N File:PDestTol2CG2.png (Sequencher screenshot showing components of pDestTol2CG2.) (current)
- 19:15, 7 February 2007 (diff | hist) . . (+19,035) . . N PDestTol2CG2 sequence (New page: <pre> >pDestTol2CG2 CACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAA TACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGC CCTTATTCCCTTTTT...)
- 19:14, 7 February 2007 (diff | hist) . . (+546) . . N PDestTol2CG2 (New page: '''Construction details'''<br> pDestTol2CG2 was made from pDestTol2CG by cutting with AfeII/NgoMI and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part o...)
- 19:12, 7 February 2007 (diff | hist) . . (0) . . m PDestTol2pA2 sequence (PDestTol2pA sequence moved to PDestTol2pA2 sequence: typo in original name)
- 19:12, 7 February 2007 (diff | hist) . . (+35) . . N PDestTol2pA sequence (PDestTol2pA sequence moved to PDestTol2pA2 sequence: typo in original name)
- 19:11, 7 February 2007 (diff | hist) . . (+28) . . PDestTol2pA2
- 19:09, 7 February 2007 (diff | hist) . . (-1) . . m List of entry and destination vectors
- 19:08, 7 February 2007 (diff | hist) . . (+84) . . List of entry and destination vectors
- 19:06, 7 February 2007 (diff | hist) . . (+56) . . N File:PDestTol2pA2.png (Sequencher screenshot showing components of pDestTol2pA.)
- 19:05, 7 February 2007 (diff | hist) . . (+16,113) . . N PDestTol2pA2 sequence (New page: <pre> >pDest-Tol2-pA_v2 CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT GGAACAAGAGT...)
- 19:00, 7 February 2007 (diff | hist) . . (+519) . . N PDestTol2pA2 (New page: '''Construction details'''<br> pDestTol2pA2 was made from pDestTol2pA by cutting with AfeII/DraIII and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part ...)
- 19:08, 6 February 2007 (diff | hist) . . (+66) . . List of entry and destination vectors
- 13:11, 2 February 2007 (diff | hist) . . (+217) . . Main Page
- 13:05, 2 February 2007 (diff | hist) . . (+104) . . Main Page
- 13:03, 2 February 2007 (diff | hist) . . (+187) . . List of entry and destination vectors
- 12:46, 2 February 2007 (diff | hist) . . (0) . . Basic Gateway principles (→Principles of Gateway recombination)
- 12:40, 2 February 2007 (diff | hist) . . (+37) . . Basic Gateway principles (→Principles of Gateway recombination)
- 12:40, 2 February 2007 (diff | hist) . . (+166) . . N File:BP reaction.png (Diagram of a Gateway BP reaction. A "middle" clone (pME) is constructed using PCR with gene-specific primers to add att sites, then recombination with a donor vector.)
- 12:39, 2 February 2007 (diff | hist) . . (+208) . . Basic Gateway principles (→Principles of Gateway recombination)
- 12:36, 2 February 2007 (diff | hist) . . (+142) . . Main Page
- 05:20, 2 February 2007 (diff | hist) . . (+52) . . Protocols (→LR reactions)
- 05:19, 2 February 2007 (diff | hist) . . (-21) . . File:Clear-opaque.png (current)
- 05:18, 2 February 2007 (diff | hist) . . (+129) . . N File:Clear-opaque.png (Image of colonies from LR reaction showing three clear (correct) colonies (arrowheads) and one opaque (incorrect) colony (arrow).)
- 19:52, 31 January 2007 (diff | hist) . . (-4) . . Basic Gateway principles
- 19:45, 31 January 2007 (diff | hist) . . (-1) . . Basic Gateway principles
- 19:35, 31 January 2007 (diff | hist) . . (-5) . . Basic Gateway principles
- 19:35, 31 January 2007 (diff | hist) . . (0) . . Basic Gateway principles
- 19:30, 31 January 2007 (diff | hist) . . (+6) . . Basic Gateway principles
- 19:16, 31 January 2007 (diff | hist) . . (-6) . . Basic Gateway principles
- 19:15, 31 January 2007 (diff | hist) . . (+18) . . Basic Gateway principles
- 19:06, 31 January 2007 (diff | hist) . . (+168) . . Basic Gateway principles
- 19:00, 31 January 2007 (diff | hist) . . (+108) . . N File:LR reaction.png (Diagram of a multisite Gateway LR reaction, combining 3 entry clones with the destination vector pDestTol2pA)
- 13:55, 31 January 2007 (diff | hist) . . (+218) . . List of entry and destination vectors
- 13:53, 31 January 2007 (diff | hist) . . (+6) . . List of entry and destination vectors
- 13:38, 31 January 2007 (diff | hist) . . (+66) . . List of entry and destination vectors
- 13:33, 31 January 2007 (diff | hist) . . (+2,506) . . N List of entry and destination vectors (New page: This is a test of table features... {| |+ '''Constructs in the Tol2kit v1 (Jan 2007)''' ! name !! # !! insert !! made by |- ! colspan="4" style="background:#ffdead;" |5' entry clones, ...)
- 17:35, 30 January 2007 (diff | hist) . . (+25) . . N References (New page: Coming some day. 1/30/07.) (current)
- 17:35, 30 January 2007 (diff | hist) . . (+30) . . N Sample results with the Tol2kit (New page: Coming at some point, 1/30/07.)
- 17:34, 30 January 2007 (diff | hist) . . (-15) . . Sources
- 17:33, 30 January 2007 (diff | hist) . . (+279) . . N Sources (New page: Coming soon 1/30/07. This page will talk about the provenance of some components that we have used in the Tol2kit: * fluorescent proteins (FPs): EGFP, mCherry * fusion tags used for FP su...)
- 17:30, 30 January 2007 (diff | hist) . . (+55) . . Main Page (→About this site)
- 17:29, 30 January 2007 (diff | hist) . . (+114) . . N Basic Gateway principles (New page: (content is coming soon. 1/30/07) == Principles of Gateway recombination == == How we use this in the Tol2kit ==)
- 17:27, 30 January 2007 (diff | hist) . . (+295) . . Main Page
- 17:23, 30 January 2007 (diff | hist) . . (-30) . . Main Page (→'''Welcome to the Tol2kit documentation site''')
- 17:22, 30 January 2007 (diff | hist) . . (-64) . . Main Page (→'''Welcome to the Tol2kit documentation site''')
- 17:21, 30 January 2007 (diff | hist) . . (+1,067) . . Main Page (→'''Welcome to the Tol2kit documentation site''')
- 04:35, 30 January 2007 (diff | hist) . . (+6) . . Main Page
- 04:35, 30 January 2007 (diff | hist) . . (+289) . . Main Page
- 03:06, 30 January 2007 (diff | hist) . . (-68) . . Main Page
- 00:35, 30 January 2007 (diff | hist) . . (+594) . . N Tol2Kit:About (New page: A multisite Gateway-based construction kit for building zebrafish expression vectors in a Tol2 transposon backbone. The constructs in the initial release of the Tol2kit (Jan 2007) were bu...) (current)